ArticleCells, tissues, organs2013
Alteration of the fibrocartilaginous nature of scaffoldless constructs formed from leporine meniscus cells and chondrocytes through manipulation of culture and processing conditions.
Article in Cells, tissues, organs, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
14 citing papers in PubMed, 26 citations in OpenAlex.
- Biomaterials and Meniscal Lesions: Current Concepts and Future Perspective.Pharmaceutics · 2021Review
- Engineering large, anatomically shaped osteochondral constructs with robust interfacial shear properties.NPJ Regenerative medicine · 2021Article
- Rejuvenation of extensively passaged human chondrocytes to engineer functional articular cartilage.Biofabrication · 2021Article
- Overcoming Challenges in Engineering Large, Scaffold-Free Neocartilage with Functional Properties.Tissue engineering. Part A · 2018Article
- Effects of passage number and post-expansion aggregate culture on tissue engineered, self-assembled neocartilage.Acta biomaterialia · 2016Article
- Cell-based tissue engineering strategies used in the clinical repair of articular cartilage.Biomaterials · 2016Review
- Initiation of Chondrocyte Self-Assembly Requires an Intact Cytoskeletal Network.Tissue engineering. Part A · 2016Article
- A preclinical evaluation of an autologous living hyaline-like cartilaginous graft for articular cartilage repair: a pilot study.Scientific reports · 2015Article
- Emergence of scaffold-free approaches for tissue engineering musculoskeletal cartilages.Annals of biomedical engineering · 2015Review
- Neocartilage integration in temporomandibular joint discs: physical and enzymatic methods.Journal of the Royal Society, Interface · 2015Article
- Harnessing biomechanics to develop cartilage regeneration strategies.Journal of biomechanical engineering · 2015Review
- Engineering a fibrocartilage spectrum through modulation of aggregate redifferentiation.Cell transplantation · 2015Article
- Enhancing post-expansion chondrogenic potential of costochondral cells in self-assembled neocartilage.PloS one · 2013Article
- Inducing articular cartilage phenotype in costochondral cells.Arthritis research & therapy · 2013Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 2 countries.
Funding
Abstract
Articular cartilage and the menisci of the knee joint lack intrinsic repair capacity; thus, injuries to these tissues result in eventual osteoarthrotic changes to the joint. Tissue engineering offers the potential to replace damaged cartilage and mitigate long-term debilitating changes to the joint. In an attempt to enhance the ability of adult articular chondrocytes (ACs) and meniscus cells (MCs) to produce robust scaffoldless neocartilage, the effects of passage number, cryopreservation, and redifferentiation prior to construct formation were studied. By increasing passage number, smaller donor biopsies could be used to generate sufficient cells for tissue engineering and, in this study, no detrimental effects were observed when employing passage-4 versus passage-3 cells. Cryopreservation of cells would enable the generation of a cell bank thus reducing lead time and enhancing consistency of cell-based therapies. Interestingly, cryopreservation was shown to enhance the biomechanical properties of the resultant self-assembled constructs. With regard to redifferentiation prior to construct formation, aggregate redifferentiation was shown to enhance the biochemical and biomechanical properties of self-assembled constructs. By increasing passaging number, cryopreserving cells, and applying aggregate redifferentiation prior to neotissue formation, the utility of ACs and MCs in tissue engineering can be enhanced.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.