ArticleMolecular pharmaceutics2013
Investigation of Polyethylenimine/DNA Polyplex Transfection to Cultured Cells Using Radiolabeling and Subcellular Fractionation Methods.
Article in Molecular pharmaceutics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
17 citing papers in PubMed.
- Sequence-defined polymers for predictable gene delivery.Nature reviews. Chemistry · 2026Review
- Lactosylated lipid calcium phosphate-based nanoparticles: A promising approach for efficient DNA delivery to hepatocytes.Iranian journal of basic medical sciences · 2024Article
- Genetically Encoded Split-Luciferase Biosensors to Measure Endosome Disruption Rapidly in Live Cells.ACS sensors · 2020Article
- Role of Cellular Retention and Intracellular State in Controlling Gene Delivery Efficiency of Multiple Nonviral Carriers.ACS omega · 2019Article
- Gal8 Visualization of Endosome Disruption Predicts Carrier-Mediated Biologic Drug Intracellular Bioavailability.ACS nano · 2019Article
- Article
- From 2D to 3D patches on multifunctional particles: how microcontact printing creates a new dimension of functionality.Soft matter · 2018Article
- Role of Cell Membrane-Vector Interactions in Successful Gene Delivery.Accounts of chemical research · 2016Article
- Cationic Polymer Intercalation into the Lipid Membrane Enables Intact Polyplex DNA Escape from Endosomes for Gene Delivery.Molecular pharmaceutics · 2016Article
- Multilayered Nanoparticles for Gene Delivery Used to Reprogram Human Foreskin Fibroblasts to Neurospheres.Tissue engineering. Part C, Methods · 2015Article
- Multivalent display of pendant pro-apoptotic peptides increases cytotoxic activity.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Kinome-level screening identifies inhibition of polo-like kinase-1 (PLK1) as a target for enhancing non-viral transgene expression.Journal of controlled release : official journal of the Controlled Release Society · 2015Article
- Histone-targeted Polyplexes Avoid Endosomal Escape and Enter the Nucleus During Postmitotic Redistribution of ER Membranes.Molecular therapy. Nucleic acids · 2015Article
- Neutral polymer micelle carriers with pH-responsive, endosome-releasing activity modulate antigen trafficking to enhance CD8(+) T cell responses.Journal of controlled release : official journal of the Controlled Release Society · 2014Article
- Effect of polyplex morphology on cellular uptake, intracellular trafficking, and transgene expression.ACS nano · 2013Article
- Engineering biodegradable and multifunctional peptide-based polymers for gene delivery.Journal of biological engineering · 2013Article
- Advancing polymeric delivery systems amidst a nucleic acid therapy renaissance.ACS macro letters · 2013Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
Quantitative analysis of the intracellular trafficking of nonviral vectors provides critical information that can guide the rational design of improved cationic systems for gene delivery. Subcellular fractionation methods, combined with radiolabeling, produce quantitative measurements of the intracellular trafficking of nonviral vectors and the therapeutic payload. In this work, differential and density-gradient centrifugation techniques were used to determine the intracellular distribution of radiolabeled 25 kDa branched polyethylenimine (bPEI)/plasmid DNA complexes ("polyplexes") in HeLa cells over time. By differential centrifugation, [(14)C]bPEI was found mostly in the lighter fractions whereas [(3)H]DNA was found mostly in the heavier fractions. A majority of the intracellular polymer (∼60%) and DNA (∼90%) were found in the nuclear fraction. Polymer and DNA also differed in their distribution to heavier and denser organelles (lysosomes, mitochondria) in density-gradient centrifugation studies. An unexpected finding from this study was that between 18 and 50% of the DNA applied to the cells became cell-associated (either with the cell membrane and/or internalized), while only 1-6% of the polymer did so, resulting in an effective N/P ratio of less than 1. These results suggest that a significant amount of cationic polymer is dissociated from the DNA cargo early on in the transfection process.
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Registered trials
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