ArticlePloS one2013
Essential role of MFG-E8 for phagocytic properties of microglial cells.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed, 39 citations in OpenAlex.
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- Phenotypic Effects of Wild-Type and Mutant SOD1 Expression in N9 Murine Microglia at Steady State, Inflammatory and Immunomodulatory Conditions.Frontiers in cellular neuroscience · 2019Article
- The Healing Effect of Human Milk Fat Globule-EGF Factor 8 Protein (MFG-E8) in A Rat Model of Parkinson's Disease.Brain sciences · 2018Article
- Downregulated Glia Interplay and Increased miRNA-155 as Promising Markers to Track ALS at an Early Stage.Molecular neurobiology · 2018Article
- MFG-E8 Selectively Inhibited Aβ-Induced Microglial M1 Polarization via NF-κB and PI3K-Akt Pathways.Molecular neurobiology · 2017Article
- A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells.The Journal of biological chemistry · 2017Article
- The many ways tissue phagocytes respond to dying cells.Immunological reviews · 2017Review
- Key Aging-Associated Alterations in Primary Microglia Response to Beta-Amyloid Stimulation.Frontiers in aging neuroscience · 2017Article
- Exploring New Inflammatory Biomarkers and Pathways during LPS-Induced M1 Polarization.Mediators of inflammation · 2016Article
- IFNβ secreted by microglia mediates clearance of myelin debris in CNS autoimmunity.Acta neuropathologica communications · 2015Article
- Development of non-viral vehicles for targeted gene transfer into microglia via the integrin receptor CD11b.Frontiers in molecular neuroscience · 2014Article
- Influence of subretinal fluid in advanced stage retinopathy of prematurity on proangiogenic response and cell proliferation.Molecular vision · 2014Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Milk fat globule factor-E8 (MFG-E8) has been regarded as a key factor involved in the phagocytosis of apoptotic cells. We induced a lentivirus into the microglial cells for the augmentation or abrogation of MFG-E8 expression in mouse microglial cells, and investigated phagocytosis of phosphatidylserine tagged human red blood cells (hRBCs) in co-cultures. Increased MFG-E8 levels were associated with a significant increase in phagocytic activity compared to the controls. Conversely, phagocytosis dramitically decreased due to the abrogation of MFG-E8. In addition, the expression of the inflammatory cytokines, TNF-α and IL-1β, also increased or decreased in the microglial cells with the augmentation or abrogation of MFG-E8, respectively. Our findings indicate that the enhanced expression of MFG-E8 could increase phagocytosis of apoptotic cells; conversely, the rate of phagocytosis and the expression of inflammatory cytokines decreased when MFG-E8 expression was knocked down. Our results confirm that MFG-E8 plays an important role in phagocytosis, and possibly serves as an essential signal molecule for microglial cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.