ArticlePloS one2013
Multidisciplinary evidences that Synechocystis PCC6803 exopolysaccharides operate in cell sedimentation and protection against salt and metal stresses.
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 58 papers.
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Who cites it
58 citing papers in PubMed, 153 citations in OpenAlex.
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- Ethylene signals through an ethylene receptor to modulate biofilm formation and root colonization in a beneficial plant-associated bacterium.PLoS genetics · 2025Article
- Improvement Schemes for Bacteria in MICP: A Review.Materials (Basel, Switzerland) · 2024Review
- Enhancing aeolian sand stability using microbially induced calcite precipitation technology.Scientific reports · 2024Article
- An Exopolysaccharide from the CyanobacteriumACS omega · 2024Review
- Interactions between the nitrogen-fixing cyanobacteriumISME communications · 2024Article
- Environmental modulation of exopolysaccharide production in the cyanobacterium Synechocystis 6803.Applied microbiology and biotechnology · 2023Article
- Cell specialization in cyanobacterial biofilm development revealed by expression of a cell-surface and extracellular matrix protein.NPJ biofilms and microbiomes · 2023Article
- Tyrosine phosphorylation controlled poly(A) polymerase I activity regulates general stress response in bacteria.Life science alliance · 2023Article
- Differential near-infrared imaging of heterocysts using single-walled carbon nanotubes.Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology · 2023Article
- Article
- The role of microplastics biofilm in accumulation of trace metals in aquatic environments.World journal of microbiology & biotechnology · 2022Review
- CRISPRi as a Tool to Repress Multiple Copies of Extracellular Polymeric Substances (EPS)-Related Genes in the CyanobacteriumLife (Basel, Switzerland) · 2021Article
- Article
- Bacterial Exopolysaccharides: Insight into Their Role in Plant Abiotic Stress Tolerance.Journal of microbiology and biotechnology · 2021Review
- Biosynthesis of a sulfated exopolysaccharide, synechan, and bloom formation in the model cyanobacteriumeLife · 2021Article
- Genetic, Genomics, and Responses to Stresses in Cyanobacteria: Biotechnological Implications.Genes · 2021Review
- Metals Alter Membership but Not Diversity of a Headwater Stream Microbiome.Applied and environmental microbiology · 2021Article
- Absence of KpsM (Slr0977) Impairs the Secretion of Extracellular Polymeric Substances (EPS) and Impacts Carbon Fluxes inmSphere · 2021Article
Corrections and comments
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Authors and funding
7 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Little is known about the production of exopolysaccharides (EPS) in cyanobacteria, and there are no genetic and physiological evidences that EPS are involved in cell protection against the frequently encountered environmental stresses caused by salt and metals. We studied four presumptive EPS production genes, sll0923, sll1581, slr1875 and sll5052, in the model cyanobacterium Synechocystis PCC6803, which produces copious amounts of EPS attached to cells (CPS) and released in the culture medium (RPS) as shown here. We show that sll0923, sll1581, slr1875 and sll5052 are all dispensable to the growth of all corresponding single and double deletion mutants in absence of stress. Furthermore, we report that sll0923, sll1581 and slr1875 unambiguously operate in the production of both CPS and RPS. Both sll1581 and slr1875 are more important than sll0923 for CPS production, whereas the contrary is true for RPS production. We show that the most EPS-depleted mutant, doubly deleted for sll1581 and slr1875, lacks the EPS mantle that surrounds WT cells and sorbs iron in their vicinity. Using this mutant, we demonstrate for the first time that cyanobacterial EPS directly operate in cell protection against NaCl, CoCl(2), CdSO(4) and Fe-starvation. We believe that our EPS-depleted mutants will be useful tools to investigate the role of EPS in cell-to-cell aggregation, biofilm formation, biomineralization and tolerance to environmental stresses. We also suggest using the fast sedimenting mutants as biotechnological cell factories to facilitate the otherwise expensive harvest of the producer cell biomass and/or its separation from products excreted in the growth media.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.