ArticleThe Journal of biological chemistry2013
Structural complexity of non-acid glycosphingolipids in human embryonic stem cells grown under feeder-free conditions.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Integrated Multi-Omics Analysis Uncovers Molecular Dysregulation in Glucosylceramide Synthase-Deficient Human Induced Pluripotent Stem Cells.Molecular & cellular proteomics : MCP · 2026Article
- Glycosphingolipids in human abdominal leiomyosarcoma and liposarcoma.The Journal of biological chemistry · 2026Article
- Characterization of the ganglioside recognition profile of Escherichia coli heat-labile enterotoxin LT-IIc.Glycobiology · 2022Article
- Characterization of Human Medullary Thyroid Carcinoma Glycosphingolipids Identifies Potential Cancer Markers.International journal of molecular sciences · 2021Article
- Characterization of Glycosphingolipids in the Human Parathyroid and Thyroid Glands.International journal of molecular sciences · 2021Article
- B3GALT5 knockout alters gycosphingolipid profile and facilitates transition to human naïve pluripotency.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Review
- Article
- Glycosphingolipids of human embryonic stem cells.Glycoconjugate journal · 2017Review
- Glycosphingolipid dynamics in human embryonic stem cell and cancer: their characterization and biomedical implications.Glycoconjugate journal · 2017Review
- HLA and Histo-Blood Group Antigen Expression in Human Pluripotent Stem Cells and their Derivatives.Scientific reports · 2017Article
- Glycolipid dynamics in generation and differentiation of induced pluripotent stem cells.Scientific reports · 2015Article
- A Cytotoxic Antibody Recognizing Lacto-N-fucopentaose I (LNFP I) on Human Induced Pluripotent Stem (hiPS) Cells.The Journal of biological chemistry · 2015Article
- Sialyl-lactotetra, a novel cell surface marker of undifferentiated human pluripotent stem cells.The Journal of biological chemistry · 2014Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Due to their pluripotency and growth capability, there are great expectations for human embryonic stem cells, both as a resource for functional studies of early human development and as a renewable source of cells for use in regenerative medicine and transplantation. However, to bring human embryonic stem cells into clinical applications, their cell surface antigen expression and its chemical structural complexity have to be defined. In the present study, total non-acid glycosphingolipid fractions were isolated from two human embryonic stem cell lines (SA121 and SA181) originating from leftover in vitro fertilized human embryos, using large amounts of starting material (1 × 10(9) cells/cell line). The total non-acid glycosphingolipid fractions were characterized by antibody and lectin binding, mass spectrometry, and proton NMR. In addition to the globo-series and type 1 core chain glycosphingolipids previously described in human embryonic stem cells, a number of type 2 core chain glycosphingolipids (neo-lactotetraosylceramide, the H type 2 pentaosylceramide, the Le(x) pentaosylceramide, and the Le(y) hexaosylceramide) were identified as well as the blood group A type 1 hexaosylceramide. Finally, the mono-, di-, and triglycosylceramides were characterized as galactosylceramide, glucosylceramide, lactosylceramide, galabiaosylceramide, globotriaosylceramide, and lactotriaosylceramide. Thus, the glycan diversity of human embryonic stem cells, including cell surface immune determinants, is more complex than previously appreciated.
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