ArticleInfection and immunity2013
Quorum-sensing systems LuxS/autoinducer 2 and Com regulate Streptococcus pneumoniae biofilms in a bioreactor with living cultures of human respiratory cells.
Article in Infection and immunity, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 47 papers.
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Who cites it
47 citing papers in PubMed, 82 citations in OpenAlex.
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- Ultrastructural, metabolic and genetic characteristics of determinants facilitating the acquisition of macrolide resistance by Streptococcus pneumoniae.Drug resistance updates : reviews and commentaries in antimicrobial and anticancer chemotherapy · 2024Article
- The quorum sensing com system regulates pneumococcal colonisation and invasive disease in a pseudo-stratified airway tissue model.Microbiological research · 2023Article
- Measuring Niche-Associated Metabolic Activity in Planktonic and Biofilm Bacteria.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Pneumolysin promotes host cell necroptosis and bacterial competence during pneumococcal meningitis as shown by whole-animal dual RNA-seq.Cell reports · 2022Article
- The Role ofPathogens (Basel, Switzerland) · 2022Article
- Prophylactic Inhibition of Colonization by Streptococcus pneumoniae with the Secondary Bile Acid Metabolite Deoxycholic Acid.Infection and immunity · 2021Article
- Comparison of specific in-vitro virulence gene expression and innate host response in locally invasive vs colonizer strains of Streptococcus pneumoniae.Medical microbiology and immunology · 2021Article
- Hemoglobin Induces Early and Robust Biofilm Development inInfection and immunity · 2021Article
- MoWa: A Disinfectant for Hospital Surfaces Contaminated With Methicillin-ResistantFrontiers in cellular and infection microbiology · 2021Article
- Deciphering Streptococcal Biofilms.Microorganisms · 2020Review
- The alternative sigma factor σeLife · 2020Article
- Designing cyclic competence-stimulating peptide (CSP) analogs with pan-group quorum-sensing inhibition activity inProceedings of the National Academy of Sciences of the United States of America · 2020Article
- Asian Sand Dust Particles Increased Pneumococcal Biofilm FormationFrontiers in genetics · 2020Article
- Clinical Relevance and Molecular Pathogenesis of the Emerging Serotypes 22F and 33F ofFrontiers in microbiology · 2020Article
- Interaction between Streptococcus pneumoniae and Staphylococcus aureus GeneratesJournal of bacteriology · 2019Article
- A Connective Tissue Mast-Cell-Specific Receptor Detects Bacterial Quorum-Sensing Molecules and Mediates Antibacterial Immunity.Cell host & microbe · 2019Article
- Inter-kingdom signaling between gut microbiota and their host.Cellular and molecular life sciences : CMLS · 2019Review
- Biofilm Formations in Pediatric Respiratory Tract Infection Part 2: Mucosal Biofilm Formation by Respiratory Pathogens and Current and Future Therapeutic Strategies to Inhibit Biofilm Formation or Eradicate Established Biofilm.Current infectious disease reports · 2019Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
Abstract
Streptococcus pneumoniae forms organized biofilms in the human upper respiratory tract that may play an essential role in both persistence and acute respiratory infection. However, the production and regulation of biofilms on human cells is not yet fully understood. In this work, we developed a bioreactor with living cultures of human respiratory epithelial cells (HREC) and a continuous flow of nutrients, mimicking the microenvironment of the human respiratory epithelium, to study the production and regulation of S. pneumoniae biofilms (SPB). SPB were also produced under static conditions on immobilized HREC. Our experiments demonstrated that the biomass of SPB increased significantly when grown on HREC compared to the amount on abiotic surfaces. Additionally, pneumococcal strains produced more early biofilms on lung cells than on pharyngeal cells. Utilizing the bioreactor or immobilized human cells, the production of early SPB was found to be regulated by two quorum-sensing systems, Com and LuxS/AI-2, since a mutation in either comC or luxS rendered the pneumococcus unable to produce early biofilms on HREC. Interestingly, while LuxS/autoinducer 2 (AI-2) regulated biofilms on both HREC and abiotic surfaces, Com control was specific for those structures produced on HREC. The biofilm phenotypes of strain D39-derivative ΔcomC and ΔluxS QS mutants were reversed by genetic complementation. Of note, SPB formed on immobilized HREC and incubated under static conditions were completely lysed 24 h postinoculation. Biofilm lysis was also regulated by the Com and LuxS/AI-2 quorum-sensing systems.
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