ArticleBiotechnology for biofuels2013
Transporter engineering for improved tolerance against alkane biofuels in Saccharomyces cerevisiae.
Article in Biotechnology for biofuels, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 115 citations in OpenAlex.
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- Identification and expression of small multidrug resistance transporters in early-branching anaerobic fungi.Protein science : a publication of the Protein Society · 2023Article
- A unique, newly discovered four-member protein family involved in extracellular fatty acid binding in Yarrowia lipolytica.Microbial cell factories · 2022Article
- Enhancing biofuels production by engineering the actin cytoskeleton in Saccharomyces cerevisiae.Nature communications · 2022Article
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- Exploring the transportome of the biosurfactant producing yeast Starmerella bombicola.BMC genomics · 2022Article
- Degradation potential of alkanes by diverse oil-degrading bacteria from deep-sea sediments of Haima cold seep areas, South China Sea.Frontiers in microbiology · 2022Article
- Transport engineering for improving the production and secretion of valuable alkaloids inMetabolic engineering communications · 2021Article
- High-efficiency production of bisabolene from waste cooking oil by metabolically engineered Yarrowia lipolytica.Microbial biotechnology · 2021Article
- Microbial production of advanced biofuels.Nature reviews. Microbiology · 2021Review
- In-depth understanding of molecular mechanisms of aldehyde toxicity to engineer robust Saccharomyces cerevisiae.Applied microbiology and biotechnology · 2021Review
- Engineering endogenous ABC transporter with improving ATP supply and membrane flexibility enhances the secretion of β-carotene inBiotechnology for biofuels · 2020Article
- Mechanism-Driven Metabolic Engineering for Bio-Based Production of FreeFrontiers in bioengineering and biotechnology · 2020Article
- Engineering energetically efficient transport of dicarboxylic acids in yeastProceedings of the National Academy of Sciences of the United States of America · 2019Article
- Engineering Saccharomyces cerevisiae cells for production of fatty acid-derived biofuels and chemicals.Open biology · 2019Review
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundHydrocarbon alkanes, components of major fossil fuels, are considered as next-generation biofuels because their biological production has recently been shown to be possible. However, high-yield alkane production requires robust host cells that are tolerant against alkanes, which exhibit cytotoxicity. In this study, we aimed to improve alkane tolerance in Saccharomyces cerevisiae, a key industrial microbial host, by harnessing heterologous transporters that potentially pump out alkanes.
resultsTo this end, we attempted to exploit ABC transporters in Yarrowia lipolytica based on the observation that it utilizes alkanes as a carbon source. We confirmed the increased transcription of ABC2 and ABC3 transporters upon exposure to a range of alkanes in Y. lipolytica. We then showed that the heterologous expression of ABC2 and ABC3 transporters significantly increased tolerance against decane and undecane in S. cerevisiae through maintaining lower intracellular alkane level. In particular, ABC2 transporter increased the tolerance limit of S. cerevisiae about 80-fold against decane. Furthermore, through site-directed mutagenesis for glutamate (E988 for ABC2, and E989 for ABC3) and histidine (H1020 for ABC2, and H1021 for ABC3), we provided the evidence that glutamate was essential for the activity of ABC2 and ABC3 transporters, with ATP most likely to be hydrolyzed by a catalytic carboxylate mechanism.
conclusionsHere, we demonstrated that transporter engineering through expression of heterologous efflux pumps led to significantly improved tolerance against alkane biofuels in S. cerevisiae. We believe that our results laid the groundwork for developing robust alkane-producing yeast cells through transporter engineering, which will greatly aid in next-generation alkane biofuel production and recovery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.