ArticleMolecular and cellular biology2013
Distinguishing core and holoenzyme mechanisms of transcription termination by RNA polymerase III.
Article in Molecular and cellular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed.
- Terminator-dependent facilitated recycling of RNA polymerase III provides gene-specific transcriptional activation in vivo.Science advances · 2026Article
- Mutational and biophysical analyses reveal a TFIIIC binding region in the TFIIF-related Rpc53 subunit of RNA polymerase III.The Journal of biological chemistry · 2023Article
- Structural basis of Ty1 integrase tethering to RNA polymerase III for targeted retrotransposon integration.Nature communications · 2023Article
- Structural insights into nuclear transcription by eukaryotic DNA-dependent RNA polymerases.Nature reviews. Molecular cell biology · 2022Review
- Evolution of the RNA Cleavage Subunit C11/RPC10, and Recycling by RNA Polymerase III.Journal of cellular immunology · 2022Article
- The nuclear and cytoplasmic activities of RNA polymerase III, and an evolving transcriptome for surveillance.Nucleic acids research · 2021Article
- Structural insights into RNA polymerase III-mediated transcription termination through trapping poly-deoxythymidine.Nature communications · 2021Article
- Mechanism of RNA polymerase III termination-associated reinitiation-recycling conferred by the essential function of the N terminal-and-linker domain of the C11 subunit.Nature communications · 2021Article
- Article
- The vault RNA ofThe Journal of biological chemistry · 2019Article
- RNA polymerase III subunits C37/53 modulate rU:dA hybrid 3' end dynamics during transcription termination.Nucleic acids research · 2019Article
- The A12.2 Subunit Is an Intrinsic Destabilizer of the RNA Polymerase I Elongation Complex.Biophysical journal · 2018Article
- Characterizing Expression and Processing of Precursor and Mature Human tRNAs by Hydro-tRNAseq and PAR-CLIP.Cell reports · 2017Article
- Factors That Shape Eukaryotic tRNAomes: Processing, Modification and Anticodon-Codon Use.Biomolecules · 2017Review
- Novel layers of RNA polymerase III control affecting tRNA gene transcription in eukaryotes.Open biology · 2017Review
- Active Center Control of Termination by RNA Polymerase III and tRNA Gene Transcription Levels In Vivo.PLoS genetics · 2016Article
- A high density of cis-information terminates RNA Polymerase III on a 2-rail track.RNA biology · 2016Article
- Mechanism of Transcription Termination by RNA Polymerase III Utilizes a Non-template Strand Sequence-Specific Signal Element.Molecular cell · 2015Article
- Review
- Biochemical analysis of transcription termination by RNA polymerase III from yeast Saccharomyces cerevisiae.Methods in molecular biology (Clifton, N.J.) · 2015Article
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2 authors.
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Abstract
Transcription termination by RNA polymerase (Pol) III serves multiple purposes; it delimits interference with downstream genes, forms 3' oligo(U) binding sites for the posttranscriptional processing factor, La protein, and resets the polymerase complex for reinitiation. Although an interplay of several Pol III subunits is known to collectively control these activities, how they affect molecular function of the active center during termination is incompletely understood. We have approached this using immobilized Pol III-nucleic acid scaffolds to examine the two major components of termination, transcription pausing and RNA release. This allowed us to distinguish two mechanisms of termination by isolated Saccharomyces cerevisiae Pol III. A core mechanism can operate in the absence of C53/37 and C11 subunits but requires synthesis of 8 or more 3' U nucleotides, apparently reflecting inherent sensitivity to an oligo(rU·dA) hybrid that is the termination signal proper. The holoenzyme mechanism requires fewer U nucleotides but uses C53/37 and C11 to slow elongation and prevent terminator arrest. N-terminal truncation of C53 or point mutations that disable the cleavage activity of C11 impair their antiarrest activities. The data are consistent with a model in which C53, C37, and C11 activities are functionally integrated with the active center of Pol III during termination.
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