ArticleProceedings of the National Academy of Sciences of the United States of America2013
Mitosis-meiosis and sperm-oocyte fate decisions are separable regulatory events.
Article in Proceedings of the National Academy of Sciences of the United States of America, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- Lessons on the force-form-function connection in cell biology from modeling a syncytial germline.Current opinion in cell biology · 2025Review
- Analysis of the C. elegans Germline Stem Cell Pool.Methods in molecular biology (Clifton, N.J.) · 2023Article
- Non-autonomous regulation of germline stem cell proliferation by somatic MPK-1/MAPK activity in C. elegans.Cell reports · 2021Article
- Two classes of active transcription sites and their roles in developmental regulation.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Diverse Roles of PUF Proteins in Germline Stem and Progenitor Cell Development inFrontiers in cell and developmental biology · 2020Review
- Dose-Dependent Effects of GLD-2 and GLD-1 on Germline Differentiation and Dedifferentiation in the Absence of PUF-8.Frontiers in cell and developmental biology · 2020Article
- MPK-1/ERK regulatory network controls the number of sperm by regulating timing of sperm-oocyte switch in C. elegans germline.Biochemical and biophysical research communications · 2017Article
- A Phenotype-Based RNAi Screening for Ras-ERK/MAPK Signaling-Associated Stem Cell Regulators in C. elegans.Methods in molecular biology (Clifton, N.J.) · 2017Article
- A systematic mRNA control mechanism for germline stem cell homeostasis and cell fate specification.BMB reports · 2016Review
- Genomic Analyses of Sperm Fate Regulator Targets Reveal a Common Set of Oogenic mRNAs in Caenorhabditis elegans.Genetics · 2016Article
- Competence for chemical reprogramming of sexual fate correlates with an intersexual molecular signature in Caenorhabditis elegans.Genetics · 2014Article
- Dependence of the sperm/oocyte decision on the nucleosome remodeling factor complex was acquired during recent Caenorhabditis briggsae evolution.Molecular biology and evolution · 2014Article
- Article
- The regulation of spermatogenesis and sperm function in nematodes.Seminars in cell & developmental biology · 2014Review
- Oocyte differentiation is genetically dissociable from meiosis in mice.Nature genetics · 2013Article
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3 authors.
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Abstract
Germ cell fate decisions are poorly understood, despite their central role in reproduction. One fundamental question has been whether germ cells are regulated to enter the meiotic cell cycle (i.e., mitosis-meiosis decision) and to be sperm or oocyte (i.e., sperm-oocyte decision) through one or two cell fate choices. If a single decision is used, a male-specific or female-specific meiotic entry would lead necessarily toward spermatogenesis or oogenesis, respectively. If two distinct decisions are used, meiotic entry should be separable from specification as sperm or oocyte. Here, we investigate the relationship of these two decisions with tools uniquely available in the nematode Caenorhabditis elegans. Specifically, we used a temperature-sensitive Notch allele to drive germ-line stem cells into the meiotic cell cycle, followed by chemical inhibition of the Ras/ERK pathway to reprogram the sperm-oocyte decision. We found that germ cells already in meiotic prophase can nonetheless be sexually transformed from a spermatogenic to an oogenic fate. This finding cleanly uncouples the mitosis-meiosis decision from the sperm-oocyte decision. In addition, we show that chemical reprogramming occurs in a germ-line region where germ cells normally transition from the mitotic to the meiotic cell cycle and that it dramatically changes the abundance of key sperm-oocyte fate regulators in meiotic germ cells. We conclude that the C. elegans mitosis-meiosis and sperm-oocyte decisions are separable regulatory events and suggest that this fundamental conclusion will hold true for germ cells throughout the animal kingdom.
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