ArticleAnesthesia and analgesia2013
Microarray analyses of genes regulated by isoflurane anesthesia in vivo: a novel approach to identifying potential preconditioning mechanisms.
Article in Anesthesia and analgesia, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed, 17 citations in OpenAlex.
- Effect of different anaesthetic techniques on gene expression profiles in patients who underwent hip arthroplasty.PloS one · 2019Trial
- Isoflurane, like sepsis, decreases CYP1A2 liver enzyme activity in intensive care patients: a clinical study and network model.Intensive care medicine experimental · 2024Article
- Longitudinal impact on rat cardiac tissue transcriptomic profiles due to acute intratracheal inhalation exposures to isoflurane.PloS one · 2021Article
- Anaesthetic-dependent changes in gene expression following acute and chronic exposure in the rodent brain.Scientific reports · 2020Article
- Isoflurane anesthesia induces liver injury by regulating the expression of insulin-like growth factor 1.Experimental and therapeutic medicine · 2017Article
- Biological processes and pathway changes in isoflurane-induced anesthesia revealed by bioinformatics analysis of gene expression profiles.Journal of anesthesia · 2015Article
- Isoflurane Ameliorates Acute Lung Injury by Preserving Epithelial Tight Junction Integrity.Anesthesiology · 2015Article
- Independent effects of sham laparotomy and anesthesia on hepatic microRNA expression in rats.BMC research notes · 2014Article
- The role of anesthetic drugs in liver apoptosis.Hepatitis monthly · 2013Review
- Acute high-level exposure to WTC particles alters expression of genes associated with oxidative stress and immune function in the lung.Journal of immunotoxicologyArticle
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
backgroundAlthough general anesthetics are recognized for their potential to render patients unconscious during surgery, exposure can also lead to long-term outcomes of both cellular damage and protection. As regards the latter, delayed anesthetic preconditioning is an evolutionarily conserved physiological response that has the potential for protecting against ischemic injury in a number of tissues. Although it is known that delayed preconditioning requires de novo protein synthesis, knowledge of anesthetic-regulated genes is incomplete. In this study, we used the conserved nature of preconditioning to analyze differentially regulated genes in 3 different rat tissues. We hypothesized that by selecting those genes regulated in multiple tissues, we could develop a focused list of gene candidates potentially involved in delayed anesthetic preconditioning.
methodsYoung adult male Sprague-Dawley rats were anesthetized with a 2% isoflurane/98% air mixture for 90 minutes. Immediately after anesthetic exposure, animals were euthanized and liver, kidney, and heart were removed and total RNA was isolated. Differential gene expression was determined using rat oligonucleotide gene arrays. Array data were analyzed to select for genes that were significantly regulated in multiple tissues.
resultsAll 3 tissues showed differentially regulated genes in response to a clinically relevant exposure to isoflurane. Analysis of coordinately regulated genes yielded a focused list of 34 potential gene candidates with a range of ontologies including regulation of inflammation, modulation of apoptosis, regulation of ion gradients, and maintenance of energy pathways.
conclusionsThrough using an analysis approach focusing on coordinately regulated genes, we were able to generate a focused list of interesting gene candidates with potential to enable future preconditioning studies.
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