ArticleArthritis and rheumatism2013
Proteomic analysis of synovial fluid from the osteoarthritic knee: comparison with transcriptome analyses of joint tissues.
Article in Arthritis and rheumatism, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 89 papers, 2 of them syntheses that pooled it.
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Who cites it
89 citing papers in PubMed, 2 syntheses or guidelines pooled it, 159 citations in OpenAlex.
- An Evidence-Based Systematic Review of Human Knee Post-Traumatic Osteoarthritis (PTOA): Timeline of Clinical Presentation and Disease Markers, Comparison of Knee Joint PTOA Models and Early Disease Implications.International journal of molecular sciences · 2021Pooled it
- Proteomics perspectives in rotator cuff research: a systematic review of gene expression and protein composition in human tendinopathy.PloS one · 2015Pooled it
- Complement Expression and Activation in Osteoarthritis Joint Compartments.Frontiers in immunology · 2020Trial
- Recent advances in understanding molecular and clinical heterogeneity in osteoarthritis: one disease or several?Osteoarthritis imaging · 2026Article
- Article
- Targeting adipocyte ESRRA alleviates osteoarthritis via interrupting inter-organelle crosstalk of complement C3-CFD-MAC cascade.Bone research · 2026Article
- APOA4 Protects Chondrocytes and Modulates Wnt Signaling in Osteoarthritis.Journal of inflammation research · 2026Article
- Spatial lipid-barrier coupling in knee osteoarthritis: a mechanism-based perspective centered on the infrapatellar fat pad.Therapeutic advances in musculoskeletal disease · 2026Review
- CRTAC1 derived from senescent FLSs induces chondrocyte mitochondrial dysfunctionActa pharmaceutica Sinica. B · 2025Article
- Synovial fluid as a complex molecular pool contributing to knee osteoarthritis.Nature reviews. Rheumatology · 2025Review
- Advancing osteoarthritis research: the role of AI in clinical, imaging and omics fields.Bone research · 2025Review
- Cholesterol metabolism and neuroinflammatory changes in a non-human primate spinal nerve ligation model.Scientific reports · 2025Article
- Biochemical markers in hand osteoarthritis: a path to precision medicine.Rheumatology international · 2025Review
- Proteomic profiling of serum in cats with naturally occurring degenerative joint disease and co-morbid conditions.Frontiers in pain research (Lausanne, Switzerland) · 2025Article
- The efficacy and safety ofHealth science reports · 2024Article
- Serum proteomic biomarkers diagnostic of knee osteoarthritis.Osteoarthritis and cartilage · 2024Article
- Proteomics Analysis of Knee Subchondral Bone Identifies Differentially Expressed Proteins Associated with Osteoarthritis.Journal of proteome research · 2024Article
- Article
- Comprehensive characterization of pathogenic synovial fluid extracellular vesicles from knee osteoarthritis.Clinical immunology (Orlando, Fla.) · 2023Article
- Human Mesenchymal Stem Cell-Derived Miniature Joint System for Disease Modeling and Drug Testing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2022Article
29 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
15 authors at 10 institutions in 2 countries.
Funding
Abstract
objectiveThe pathophysiology of the most common joint disease, osteoarthritis (OA), remains poorly understood. Since synovial fluid (SF) bathes joint cartilage and synovium, we reasoned that a comparative analysis of its protein constituents in health and OA could identify pathways involved in joint damage. We undertook this study to perform a proteomic analysis of knee SF from OA patients and control subjects and to compare the results to microarray expression data from cartilage and synovium.
methodsAge-matched knee SF samples from 10 control subjects, 10 patients with early-stage OA, and 10 patients with late-stage OA were compared using 2-dimensional difference-in-gel electrophoresis and mass spectrometry (MS). MS with a multiplexed peptide selected reaction monitoring assay was used to confirm differential expression of a subset of proteins in an independent OA patient cohort. Proteomic results were analyzed by Ingenuity Pathways Analysis and compared to published synovial tissue and cartilage messenger RNA profiles.
resultsSixty-six proteins were differentially present in healthy and OA SF. Three major pathways were identified among these proteins: the acute-phase response signaling pathway, the complement pathway, and the coagulation pathway. Differential expression of 5 proteins was confirmed by selected reaction monitoring assay. A focused analysis of transcripts corresponding to the differentially present proteins indicated that both synovial and cartilage tissues may contribute to the OA SF proteome.
conclusionProteins involved in the acute-phase response signaling pathway, the complement pathway, and the coagulation pathway are differentially regulated in SF from OA patients, suggesting that they contribute to joint damage. Validation of these pathways and their utility as biomarkers or therapeutic targets in OA is warranted.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.