ArticleLaboratory investigation; a journal of technical methods and pathology2013
Quantitation of fixative-induced morphologic and antigenic variation in mouse and human breast cancers.
Article in Laboratory investigation; a journal of technical methods and pathology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
26 citing papers in PubMed, 39 citations in OpenAlex.
- Evaluation of Glyoxal fixation for immunohistochemistry of the retina.Scientific reports · 2025Article
- EYA3 regulation of NF-κB and CCL2 suppresses cytotoxic NK cells in the premetastatic niche to promote TNBC metastasis.Science advances · 2025Article
- In situ T-cell transfection by anti-CD3-conjugated lipid nanoparticles leads to T-cell activation, migration, and phenotypic shift.Biomaterials · 2022Article
- Tumor-specific delivery of gemcitabine with activatable liposomes.Journal of controlled release : official journal of the Controlled Release Society · 2019Article
- Combining activatable nanodelivery with immunotherapy in a murine breast cancer model.Journal of controlled release : official journal of the Controlled Release Society · 2019Article
- A Syngeneic ErbB2 Mammary Cancer Model for Preclinical Immunotherapy Trials.Journal of mammary gland biology and neoplasia · 2019Article
- Digital Microscopy, Image Analysis, and Virtual Slide Repository.ILAR journal · 2018Article
- CD8Clinical cancer research : an official journal of the American Association for Cancer Research · 2018Article
- Principles and approaches for reproducible scoring of tissue stains in research.Laboratory investigation; a journal of technical methods and pathology · 2018Review
- Sphingosine-1-Phosphate Signaling and Metabolism Gene Signature in Pediatric Inflammatory Bowel Disease: A Matched-case Control Pilot Study.Inflammatory bowel diseases · 2018Article
- Distinct immune signatures in directly treated and distant tumors result from TLR adjuvants and focal ablation.Theranostics · 2018Article
- Pathobiology of the 129:Stat1Breast cancer research : BCR · 2017Article
- Priming is key to effective incorporation of image-guided thermal ablation into immunotherapy protocols.JCI insight · 2017Article
- In vitro characterization and in vivo ultrasound molecular imaging of nucleolin-targeted microbubbles.Biomaterials · 2017Article
- Intracellular trafficking of a pH-responsive drug metal complex.Journal of controlled release : official journal of the Controlled Release Society · 2016Article
- The Trp53 delta proline (Trp53ΔP) mouse exhibits increased genome instability and susceptibility to radiation-induced, but not spontaneous, tumor development.Molecular carcinogenesis · 2016Article
- Ultrasound ablation enhances drug accumulation and survival in mammary carcinoma models.The Journal of clinical investigation · 2016Article
- 184AA3: a xenograft model of ER+ breast adenocarcinoma.Breast cancer research and treatment · 2016Article
- Introduction of Zinc-salt Fixation for Effective Detection of Immune Cell-related Markers by Immunohistochemistry.Toxicologic pathology · 2015Article
- Ultrasound molecular imaging of tumor angiogenesis with a neuropilin-1-targeted microbubble.Biomaterials · 2015Article
Corrections and comments
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Authors and funding
13 authors at 2 institutions in 1 country.
Funding
Abstract
Quantitative Image Analysis (QIA) of digitized whole slide images for morphometric parameters and immunohistochemistry of breast cancer antigens was used to evaluate the technical reproducibility, biological variability, and intratumoral heterogeneity in three transplantable mouse mammary tumor models of human breast cancer. The relative preservation of structure and immunogenicity of the three mouse models and three human breast cancers was also compared when fixed with representatives of four distinct classes of fixatives. The three mouse mammary tumor cell models were an ER+/PR+ model (SSM2), a Her2+ model (NDL), and a triple negative model (MET1). The four breast cancer antigens were ER, PR, Her2, and Ki67. The fixatives included examples of (1) strong cross-linkers, (2) weak cross-linkers, (3) coagulants, and (4) combination fixatives. Each parameter was quantitatively analyzed using modified Aperio Technologies ImageScope algorithms. Careful pre-analytical adjustments to the algorithms were required to provide accurate results. The QIA permitted rigorous statistical analysis of results and grading by rank order. The analyses suggested excellent technical reproducibility and confirmed biological heterogeneity within each tumor. The strong cross-linker fixatives, such as formalin, consistently ranked higher than weak cross-linker, coagulant and combination fixatives in both the morphometric and immunohistochemical parameters.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.