ArticleMolecular & cellular proteomics : MCP2013
Spatio-temporal analysis of molecular determinants of neuronal degeneration in the aging mouse cerebellum.
Article in Molecular & cellular proteomics : MCP, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 34 citations in OpenAlex.
- Cerebellum and Aging: Update and Challenges.Aging and disease · 2024Review
- Aging, Neurodegenerative Disorders, and Cerebellum.International journal of molecular sciences · 2024Review
- Functional bioengineered tissue models of neurodegenerative diseases.Biomaterials · 2023Review
- Article
- Article
- Purkinje-cell-specific DNA repair-deficient mice reveal that dietary restriction protects neurons by cell-intrinsic preservation of genomic health.Frontiers in aging neuroscience · 2022Article
- In vivo 5-ethynyluridine (EU) labelling detects reduced transcription in Purkinje cell degeneration mouse mutants, but can itself induce neurodegeneration.Acta neuropathologica communications · 2021Article
- Biophysical and morphological changes in inner hair cells and their efferent innervation in the ageing mouse cochlea.The Journal of physiology · 2021Article
- Innovations in 3-Dimensional Tissue Models of Human Brain Physiology and Diseases.Advanced functional materials · 2020Article
- Establishment of Dimethyl Labeling-based Quantitative Acetylproteomics in Arabidopsis.Molecular & cellular proteomics : MCP · 2018Article
- An expandable embryonic stem cell-derived Purkinje neuron progenitor population that exhibits in vivo maturation in the adult mouse cerebellum.Scientific reports · 2017Article
- Restricted diet delays accelerated ageing and genomic stress in DNA-repair-deficient mice.Nature · 2016Article
- DNA Damage: From Chronic Inflammation to Age-Related Deterioration.Frontiers in genetics · 2016Review
- Cell-autonomous progeroid changes in conditional mouse models for repair endonuclease XPG deficiency.PLoS genetics · 2014Article
- Macromolecular complexes at active zones: integrated nano-machineries for neurotransmitter release.Cellular and molecular life sciences : CMLS · 2014Review
- Phosphoproteome dynamics in onset and maintenance of oncogene-induced senescence.Molecular & cellular proteomics : MCP · 2014Article
- Alterations in the cerebellar (Phospho)proteome of a cyclic guanosine monophosphate (cGMP)-dependent protein kinase knockout mouse.Molecular & cellular proteomics : MCP · 2014Article
- Supraphysiological doses of performance enhancing anabolic-androgenic steroids exert direct toxic effects on neuron-like cells.Frontiers in cellular neuroscience · 2013Article
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Authors and funding
9 authors at 4 institutions in 2 countries.
Funding
Abstract
The accumulation of cellular damage, including DNA damage, is hypothesized to contribute to aging-related neurodegenerative changes. DNA excision repair cross-complementing group 1 (Ercc1) knock-out mice represent an accepted model of neuronal aging, showing gradual neurodegenerative changes, including loss of synaptic contacts and cell body shrinkage. Here, we used the Purkinje cell-specific Ercc1 DNA-repair knock-out mouse model to study aging in the mouse cerebellum. We performed an in-depth quantitative proteomics analysis, using stable isotope dimethyl labeling, to decipher changes in protein expression between the early (8 weeks), intermediate (16 weeks), and late (26 weeks) stages of the phenotypically aging Ercc1 knock-out and healthy littermate control mice. The expression of over 5,200 proteins from the cerebellum was compared quantitatively, whereby 79 proteins (i.e. 1.5%) were found to be substantially regulated during aging. Nearly all of these molecular markers of the early aging onset belonged to a strongly interconnected network involved in excitatory synaptic signaling. Using immunohistological staining, we obtained temporal and spatial profiles of these markers confirming not only the proteomics data but in addition revealed how the change in protein expression correlates to synaptic changes in the cerebellum. In summary, this study provides a highly comprehensive spatial and temporal view of the dynamic changes in the cerebellum and Purkinje cell signaling in particular, indicating that synapse signaling is one of the first processes to be affected in this premature aging model, leading to neuron morphological changes, neuron degeneration, inflammation, and ultimately behavior disorders.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.