ArticleStem cells and development2013
Regulatory microRNA network identification in bovine blastocyst development.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 54 citations in OpenAlex.
- Gene regulation by non-Coding RNAs in infertility: a mechanistic review.Journal of ovarian research · 2025Review
- Role of miRNAs in Bovine Oocyte Maturation and Reproductive Regulation.International journal of molecular sciences · 2025Review
- Mir-218-5p from Extracellular Vesicles of Endometrium in Patients with Recurrent Implantation Failure Impairs Pre-Implantation Embryo Development.International journal of nanomedicine · 2025Article
- Sexual dimorphic miRNA-mediated response of bovine elongated embryos to the maternal microenvironment.PloS one · 2024Article
- MiR-145 is upregulated in the retarded preimplantation embryos and modulates cholesterol levels in mice preimplantation embryos through targeting Abca1.Reproductive biology and endocrinology : RB&E · 2022Article
- Embryonic microRNAs are essential for bovine preimplantation embryo development.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Interspecific Variation in One-Carbon Metabolism within the Ovarian Follicle, Oocyte, and Preimplantation Embryo: Consequences for Epigenetic Programming of DNA Methylation.International journal of molecular sciences · 2021Article
- Transient exposure to miR-203 enhances the differentiation capacity of established pluripotent stem cells.The EMBO journal · 2020Article
- The Role of MicroRNAs in Mammalian Fertility: From Gametogenesis to Embryo Implantation.International journal of molecular sciences · 2020Review
- Identification and Profiling of MicroRNAs During Embryogenesis in the Red Claw CrayfishFrontiers in physiology · 2020Article
- Bta-miR-10b Secreted by Bovine Embryos Negatively Impacts Preimplantation Embryo Quality.Frontiers in genetics · 2019Article
- Bovine Embryo-Secreted microRNA-30c Is a Potential Non-invasive Biomarker for Hampered Preimplantation Developmental Competence.Frontiers in genetics · 2019Article
- Extracellular Vesicles: Decoding a New Language for Cellular Communication in Early Embryonic Development.Frontiers in cell and developmental biology · 2018Review
- Sperm-borne miR-449b influences cleavage, epigenetic reprogramming and apoptosis of SCNT embryos in bovine.Scientific reports · 2017Article
- MicroRNA Signaling in Embryo Development.Biology · 2017Review
- MicroRNA-130b is involved in bovine granulosa and cumulus cells function, oocyte maturation and blastocyst formation.Journal of ovarian research · 2017Article
- miR-27a and miR-449b polymorphisms associated with a risk of idiopathic recurrent pregnancy loss.PloS one · 2017Article
- Human Embryos Created by Embryo Splitting Secrete Significantly Lower Levels of miRNA-30c.Stem cells and development · 2016Article
- Article
- Identification of serum microRNA signatures for diagnosis of mild traumatic brain injury in a closed head injury model.PloS one · 2014Article
Corrections and comments
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Authors and funding
8 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Mammalian blastocyst formation is characterized by two lineage segregations resulting in the formation of the trophectoderm, the hypoblast, and the epiblast cell lineages. Cell fate determination during these early lineage segregations is associated with changes in the expression of specific transcription factors. In addition to the transcription factor-based control, it has become clear that also microRNAs (miRNAs) play an important role in the post-transcriptional regulation of pluripotency and differentiation. To elucidate the role of miRNAs in early lineage segregation, we compared the miRNA expression in early bovine blastocysts with the more advanced stage of hatched blastocysts. Reverse transcription-quantitative PCR-based miRNA expression profiling revealed eight upregulated miRNAs (miR-127, miR-130a, miR-155, miR-196a, miR-203, miR-28, miR-29c, and miR-376a) and four downregulated miRNAs (miR-135a, miR-218, miR-335, and miR-449b) in hatched blastocysts. Through an integrative analysis of matching miRNA and mRNA expression data, candidate miRNA-mRNA interaction pairs were prioritized for validation. Using an in vitro luciferase reporter assay, we confirmed a direct interaction between miR-218 and CDH2, miR-218 and NANOG, and miR-449b and NOTCH1. By interfering with the FGF signaling pathway, we found functional evidence that miR-218, mainly expressed in the inner cell mass, regulates the NANOG expression in the bovine blastocyst in response to FGF signaling. The results of this study expand our knowledge about the miRNA signature of the bovine blastocyst and of the interactions between miRNAs and cell fate regulating transcription factors.
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Registered trials
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