ArticleEukaryotic cell2013
Cysteine protease inhibitor (AcStefin) is required for complete cyst formation of Acanthamoeba.
Article in Eukaryotic cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 31 citations in OpenAlex.
- Smolstatin1, a unique cystatin-like stefin ofCurrent research in microbial sciences · 2026Article
- Induction of Programmed Cell Death inAntioxidants (Basel, Switzerland) · 2023Article
- Review
- Proteases as Therapeutic Targets Against the Parasitic CnidarianFrontiers in cellular and infection microbiology · 2021Article
- Drug Discovery againstPathogens (Basel, Switzerland) · 2020Review
- Molecular and Biochemical Properties of a Cysteine Protease of Acanthamoeba castellanii.The Korean journal of parasitology · 2018Article
- Killing of diverse eye pathogens (Acanthamoeba spp., Fusarium solani, and Chlamydia trachomatis) with alcohols.PLoS neglected tropical diseases · 2017Article
- Identification of Protein Arginine Methyltransferase 5 as a Regulator for Encystation of Acanthamoeba.The Korean journal of parasitology · 2016Article
- RNA-Binding Proteins in Trichomonas vaginalis: Atypical Multifunctional Proteins.Biomolecules · 2015Review
- Trichomonas vaginalis Cysteine Proteinases: Iron Response in Gene Expression and Proteolytic Activity.BioMed research international · 2015Review
- Essential Role for an M17 Leucine Aminopeptidase in Encystation of Acanthamoeba castellanii.PloS one · 2015Article
- Chloroquine has a cytotoxic effect on Acanthamoeba encystation through modulation of autophagy.Antimicrobial agents and chemotherapy · 2014Article
- Identification of atg8 isoform in encysting Acanthamoeba.The Korean journal of parasitology · 2013Article
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Authors and funding
11 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The encystation of Acanthamoeba leads to the formation of resilient cysts from vegetative trophozoites. This process is essential for parasite survival under unfavorable conditions, such as those associated with starvation, low temperatures, and biocides. Furthermore, cysteine proteases have been implicated in the massive turnover of intracellular components required for encystation. Thus, strict modulation of the activities of cysteine proteases is required to protect Acanthamoeba from intracellular damage. However, mechanisms underlying the control of protease activity during encystation have not been established in Acanthamoeba. In the present study, we identified and characterized Acanthamoeba cysteine protease inhibitor (AcStefin), which was found to be highly expressed during encystation and to be associated with lysosomes by fluorescence microscopy. Recombinant AcStefin inhibited various cysteine proteases, including human cathepsin B, human cathepsin L, and papain. Transfection with small interfering RNA against AcStefin increased cysteine protease activity during encystation and resulted in incomplete cyst formation, reduced excystation efficiency, and a significant reduction in cytoplasmic area. Taken together, these results indicate that AcStefin is involved in the modulation of cysteine proteases and that it plays an essential role during the encystation of Acanthamoeba.
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