ArticleMicrobial ecology2013
Microbiological survey of the human gastric ecosystem using culturing and pyrosequencing methods.
Article in Microbial ecology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 124 papers, 2 of them syntheses that pooled it.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
124 citing papers in PubMed, 2 syntheses or guidelines pooled it.
- Meta-analysis of mucosal microbiota reveals universal microbial signatures and dysbiosis in gastric carcinogenesis.Oncogene · 2022Pooled it
- Pooled it
- Gastric Microbiota Dysbiosis and Microbiome-Based Interventions in Chronic Atrophic Gastritis.Nutrients · 2026Review
- The gastric ecosystem: assessing resident microbiome vs. transient microorganisms in obesity and following bariatric interventions.NPJ biofilms and microbiomes · 2026Review
- Effects of Helicobacter pylori infection on gastric mucosal microbiota.World journal of pediatrics : WJP · 2026Review
- Characterization of the Gastric Antrum Microbiome inPathogens (Basel, Switzerland) · 2026Article
- The Gut-Heart Axis: A Comprehensive Review of Microbiota's Role in Cardiovascular Health and Disease and Emerging Therapeutic Strategies.Cardiology research and practice · 2026Review
- The specific hallmarks, emerging roles, key mechanisms, and clinical applications of intra-tumoral microbiota in human cancers.Genes & diseases · 2026Review
- Pathogenicity ofWorld journal of clinical oncology · 2025Review
- Comparison of gastric mucosa microbiota in metabolically healthy and unhealthy obesity.Diabetology & metabolic syndrome · 2025Article
- Intestinal pH: a major driver of human gut microbiota composition and metabolism.Nature reviews. Gastroenterology & hepatology · 2025Review
- Review
- UnravelingVaccines · 2025Review
- Microbial Interconnections in One Health: A Critical Nexus Between Companion Animals and Human Microbiomes.Microorganisms · 2025Review
- Review
- Helicobacter pylori, microbiota and gastric cancer - principles of microorganism-driven carcinogenesis.Nature reviews. Gastroenterology & hepatology · 2025Review
- Analysis of garlic intake on atrophic gastritis risk in different infectious states of Helicobacter pylori in a case-control study.Scientific reports · 2025Article
- Review
- Link Between Oral and Gut Microbiomes: The Oral-Gut Axis.Advances in experimental medicine and biology · 2025Review
- Nutrition ofEcoSal Plus · 2024Review
64 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Stomach mucosa biopsies and gastric juices samples of 12 healthy persons were analysed by culturing in selective- and non-selective-rich media. Microbial DNA from four mucosal samples was also amplified by nested PCR using universal bacterial primers, and the 16S rDNA amplicons pyrosequenced. The total number of cultivable microorganisms recovered from the samples ranged from 10(2) to 10(4) cfu/g or ml. The isolates were identified at the species level by PCR amplification and sequencing of the 16S rDNA. Isolates belonged mainly to four genera; Propionibacterium, Lactobacillus, Streptococcus and Staphylococcus. A total of 15,622 high-quality 16S rDNA sequence reads were obtained by pyrosequencing from the four mucosal samples. Sequence analysis grouped the reads into 59 families and 69 genera, revealing wide bacterial diversity. Considerable differences in the composition of the gastric microbiota were observed among the subjects, although in all samples the most abundant operational taxonomic units belonged to Streptococcus, Propionibacterium and Lactobacillus. Comparison of the stomach microbiota with that present in other parts of the human gastrointestinal tract revealed distinctive microbial communities. This is the first study in which a combination of culture and culture-independent techniques has been used to explore the bacterial diversity of the human stomach.
Indexed as
Identifiers
23397369What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.