ArticlePflugers Archiv : European journal of physiology2013
Characterization of distinct single-channel properties of Ca²⁺ inward currents in mitochondria.
Article in Pflugers Archiv : European journal of physiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed.
- MCU-independent CaScientific reports · 2024Article
- Review
- MCU proteins dominate in vivo mitochondrial Ca2+ uptake in Arabidopsis roots.The Plant cell · 2022Article
- Mitochondrial calcium exchange in physiology and disease.Physiological reviews · 2022Review
- Sarcoplasmic reticulum-mitochondria communication; implications for cardiac arrhythmia.Journal of molecular and cellular cardiology · 2021Review
- The pore-forming subunit MCU of the mitochondrial CaDiabetologia · 2020Article
- Why don't mice lacking the mitochondrial CaThe Journal of physiology · 2020Review
- Deletion of mitochondrial calcium uniporter incompletely inhibits calcium uptake and induction of the permeability transition pore in brain mitochondria.The Journal of biological chemistry · 2018Article
- The mitochondrial calcium uniporter in the heart: energetics and beyond.The Journal of physiology · 2017Review
- UCP3 Regulates Single-Channel Activity of the Cardiac mCa1.The Journal of membrane biology · 2016Article
- Interplay Between Intracellular Ca(2+) Oscillations and Ca(2+)-stimulated Mitochondrial Metabolism.Scientific reports · 2016Article
- Article
- Fine tuning of cytosolic Ca (2+) oscillations.F1000Research · 2016Review
- UCP2 modulates single-channel properties of a MCU-dependent Ca(2+) inward current in mitochondria.Pflugers Archiv : European journal of physiology · 2015Article
- Structure and function of the mitochondrial calcium uniporter complex.Biochimica et biophysica acta · 2015Review
- The regulation of neuronal mitochondrial metabolism by calcium.The Journal of physiology · 2015Review
- The Mitochondrial Calcium Uniporter Selectively Matches Metabolic Output to Acute Contractile Stress in the Heart.Cell reports · 2015Article
- Molecular mechanism of mitochondrial calcium uptake.Cellular and molecular life sciences : CMLS · 2015Review
- The mitochondrial calcium uniporter: mice can live and die without it.Journal of molecular and cellular cardiology · 2015Review
- Mitochondrial Ca(2+) uniporter (MCU)-dependent and MCU-independent Ca(2+) channels coexist in the inner mitochondrial membrane.Pflugers Archiv : European journal of physiology · 2014Article
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4 authors.
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Abstract
Previous studies have demonstrated several molecularly distinct players involved in mitochondrial Ca(2+) uptake. In the present study, electrophysiological recordings on mitoplasts that were isolated from HeLa cells were performed in order to biophysically and pharmacologically characterize Ca(2+) currents across the inner mitochondrial membrane. In mitoplast-attached configuration with 105 mM Ca(2+) as a charge carrier, three distinct channel conductances of 11, 23, and 80 pS were observed. All types of mitochondrial currents were voltage-dependent and essentially depended on the presence of Ca(2+) in the pipette. The 23 pS channel exhibited burst kinetics. Though all channels were sensitive to ruthenium red, their sensitivity was different. The 11 and 23 pS channels exhibited a lower sensitivity to ruthenium red than the 80 pS channel. The activities of all channels persisted in the presence of cylosporin A, CGP 37187, various K(+)-channel inhibitors, and Cl(-) channel blockers disodium 4,4'-diisothiocyanatostilbene-2,2'-disulfonate and niflumic acid. Collectively, our data identified multiple conductances of Ca(2+) currents in mitoplasts isolated from HeLa cells, thus challenging the dogma of only one unique mitochondrial Ca(2+) uniporter.
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