ArticleThe Journal of biological chemistry2013
The stringency of start codon selection in the filamentous fungus Neurospora crassa.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 54 citations in OpenAlex.
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- Upstream open reading frames: new players in the landscape of cancer gene regulation.NAR cancer · 2024Review
- Regulation of TRI5 expression and deoxynivalenol biosynthesis by a long non-coding RNA in Fusarium graminearum.Nature communications · 2024Article
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- Increased levels of eIF2A inhibit translation by sequestering 40S ribosomal subunits.Nucleic acids research · 2023Article
- Translational regulation by uORFs and start codon selection stringency.Genes & development · 2023Review
- Selection of start codon during mRNA scanning in eukaryotic translation initiation.Communications biology · 2022Article
- Machine learning predicts translation initiation sites in neurologic diseases with nucleotide repeat expansions.PloS one · 2022Article
- Combinatorial analysis of translation dynamics reveals eIF2 dependence of translation initiation at near-cognate codons.Nucleic acids research · 2021Article
- Free energy landscape of RNA binding dynamics in start codon recognition by eukaryotic ribosomal pre-initiation complex.PLoS computational biology · 2021Article
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- Alt-RPL36 downregulates the PI3K-AKT-mTOR signaling pathway by interacting with TMEM24.Nature communications · 2021Article
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- Conserved Upstream Open Reading Frame Nascent Peptides That Control Translation.Annual review of genetics · 2020Review
- Prevalence of alternative AUG and non-AUG translation initiators and their regulatory effects across plants.Genome research · 2020Article
- Adaptation of codon usage to tRNA I34 modification controls translation kinetics and proteome landscape.PLoS genetics · 2020Article
- Non-AUG start codons: Expanding and regulating the small and alternative ORFeome.Experimental cell research · 2020Review
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
In eukaryotic cells initiation may occur from near-cognate codons that differ from AUG by a single nucleotide. The stringency of start codon selection impacts the efficiency of initiation at near-cognate codons and the efficiency of initiation at AUG codons in different contexts. We used a codon-optimized firefly luciferase reporter initiated with AUG or each of the nine near-cognate codons in preferred context to examine the stringency of start codon selection in the model filamentous fungus Neurospora crassa. In vivo results indicated that the hierarchy of initiation at start codons in N. crassa (AUG ≫ CUG > GUG > ACG > AUA ≈ UUG > AUU > AUC) is similar to that in human cells. Similar results were obtained by translating mRNAs in a homologous N. crassa in vitro translation system or in rabbit reticulocyte lysate. We next examined the efficiency of initiation at AUG, CUG, and UUG codons in different contexts in vitro. The preferred context was more important for efficient initiation from near-cognate codons than from AUG. These studies demonstrated that near-cognate codons are used for initiation in N. crassa. Such events could provide additional coding capacity or have regulatory functions. Analyses of the 5'-leader regions in the N. crassa transcriptome revealed examples of highly conserved near-cognate codons in preferred contexts that could extend the N termini of the predicted polypeptides.
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