ArticleJournal of applied crystallography2013
A high-pressure cryocooling method for protein crystals and biological samples with reduced background X-ray scatter.
Article in Journal of applied crystallography, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
14 citing papers in PubMed, 35 citations in OpenAlex.
- Conformational flexibility of His200 enables catalytic activity in the T200H mutant of carbonic anhydrase II.Molecules and cells · 2025Article
- A continuum of amorphous ices between low-density and high-density amorphous ice.Communications chemistry · 2024Article
- The Importance of Nuclear Quantum Effects on the Thermodynamic and Structural Properties of Low-Density Amorphous Ice: A Comparison with Hexagonal Ice.The journal of physical chemistry. B · 2023Article
- The role of high-density and low-density amorphous ice on biomolecules at cryogenic temperatures: a case study with polyalanine.Physical chemistry chemical physics : PCCP · 2021Article
- Article
- Elucidating the role of metal ions in carbonic anhydrase catalysis.Nature communications · 2020Article
- Characterizing metal-binding sites in proteins with X-ray crystallography.Nature protocols · 2018Article
- Reduction of lattice disorder in protein crystals by high-pressure cryocooling.Journal of applied crystallography · 2016Article
- Carbon Dioxide "Trapped" in a β-Carbonic Anhydrase.Biochemistry · 2015Article
- In situ X-ray data collection and structure phasing of protein crystals at Structural Biology Center 19-ID.Journal of synchrotron radiation · 2015Article
- Glass-to-cryogenic-liquid transitions in aqueous solutions suggested by crack healing.Proceedings of the National Academy of Sciences of the United States of America · 2015Article
- Practical macromolecular cryocrystallography.Acta crystallographica. Section F, Structural biology communications · 2015Review
- High-resolution X-ray crystal structure of bovine H-protein using the high-pressure cryocooling method.Journal of synchrotron radiation · 2013Article
- Graphene as a protein crystal mounting material to reduce background scatter.Journal of applied crystallography · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 1 country.
Funding
Abstract
High-pressure cryocooling has been developed as an alternative method for cryopreservation of macromolecular crystals and successfully applied for various technical and scientific studies. The method requires the preservation of crystal hydration as the crystal is pressurized with dry helium gas. Previously, crystal hydration was maintained either by coating crystals with a mineral oil or by enclosing crystals in a capillary which was filled with crystallization mother liquor. These methods are not well suited to weakly diffracting crystals because of the relatively high background scattering from the hydrating materials. Here, an alternative method of crystal hydration, called capillary shielding, is described. The specimen is kept hydrated via vapor diffusion in a shielding capillary while it is being pressure cryocooled. After cryocooling, the shielding capillary is removed to reduce background X-ray scattering. It is shown that, compared to previous crystal-hydration methods, the new hydration method produces superior crystal diffraction with little sign of crystal damage. Using the new method, a weakly diffracting protein crystal may be properly pressure cryo-cooled with little or no addition of external cryoprotectants, and significantly reduced background scattering can be observed from the resulting sample. Beyond the applications for macromolecular crystallography, it is shown that the method has great potential for the preparation of noncrystalline hydrated biological samples for coherent diffraction imaging with future X-ray sources.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.