ArticleCellular & molecular immunology2013
Human immune cells' behavior and survival under bioenergetically restricted conditions in an in vitro fracture hematoma model.
Article in Cellular & molecular immunology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 52 citations in OpenAlex.
- Aptamer-functionalized EGCG nanocarrier for neutrophil-targeted AKBA delivery promotes anxiety-related fracture healing.Bioactive materials · 2027Article
- Temporal Molecular Signatures of Early Human Clavicle Fracture Healing: Characterization of Hematological, Cytokine, and miRNA Profiles.International journal of molecular sciences · 2025Article
- Research progress of gene therapy combined with tissue engineering to promote bone regeneration.APL bioengineering · 2024Review
- Temporal dynamics of immune-stromal cell interactions in fracture healing.Frontiers in immunology · 2024Review
- Enhancement of Immunosuppressive Activity of Mesenchymal Stromal Cells by Platelet-Derived Factors is Accompanied by Apoptotic Priming.Stem cell reviews and reports · 2023Article
- The benefits of adipocyte metabolism in bone health and regeneration.Frontiers in cell and developmental biology · 2023Review
- [The DRFZ-a pioneer in research on the interaction between immune and stromal cells during de- and regeneration of the musculoskeletal system].Zeitschrift fur Rheumatologie · 2022Review
- Targeting CD38 in Neoplasms and Non-Cancer Diseases.Cancers · 2022Review
- Postoperative Fluid Collections in Total Joint Arthroplasty: A Narrative Review.Orthopedic research and reviews · 2022Review
- Fracture Healing Research-Shift towards In Vitro Modeling?Biomedicines · 2021Review
- Production of IL-6 and Phagocytosis Are the Most Resilient Immune Functions in Metabolically Compromised Human Monocytes.Frontiers in immunology · 2021Article
- Multi-Omics Profiling Identifies Pathways Associated With CD8Frontiers in genetics · 2021Article
- Inflammatory Profile and Osteogenic Potential of Fracture Haematoma in Humans.Journal of clinical medicine · 2019Article
- Defective Proliferation and Osteogenic Potential with Altered Immunoregulatory phenotype of Native Bone marrow-Multipotential Stromal Cells in Atrophic Fracture Non-Union.Scientific reports · 2019Article
- Hypoxia and mesenchymal stromal cells as key drivers of initial fracture healing in an equine in vitro fracture hematoma model.PloS one · 2019Article
- CTLA-4 Mediates Inhibitory Function of Mesenchymal Stem/Stromal Cells.International journal of molecular sciences · 2018Article
- Clinical and Research Approaches to Treat Non-union Fracture.Current osteoporosis reports · 2018Review
- Bone Fracture Acute Phase Response-A Unifying Theory of Fracture Repair: Clinical and Scientific Implications.Clinical reviews in bone and mineral metabolism · 2018Article
- Development of an Accurate and Proactive Immunomodulatory Strategy to Improve Bone Substitute Material-Mediated Osteogenesis and Angiogenesis.Theranostics · 2018Article
- The haematoma and its role in bone healing.Journal of experimental orthopaedics · 2017Review
Corrections and comments
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Authors and funding
15 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The initial inflammatory phase of bone fracture healing represents a critical step for the outcome of the healing process. However, both the mechanisms initiating this inflammatory phase and the function of immune cells present at the fracture site are poorly understood. In order to study the early events within a fracture hematoma, we established an in vitro fracture hematoma model: we cultured hematomas forming during an osteotomy (artificial bone fracture) of the femur during total hip arthroplasty (THA) in vitro under bioenergetically controlled conditions. This model allowed us to monitor immune cell populations, cell survival and cytokine expression during the early phase following a fracture. Moreover, this model enabled us to change the bioenergetical conditions in order to mimic the in vivo situation, which is assumed to be characterized by hypoxia and restricted amounts of nutrients. Using this model, we found that immune cells adapt to hypoxia via the expression of angiogenic factors, chemoattractants and pro-inflammatory molecules. In addition, combined restriction of oxygen and nutrient supply enhanced the selective survival of lymphocytes in comparison with that of myeloid derived cells (i.e., neutrophils). Of note, non-restricted bioenergetical conditions did not show any similar effects regarding cytokine expression and/or different survival rates of immune cell subsets. In conclusion, we found that the bioenergetical conditions are among the crucial factors inducing the initial inflammatory phase of fracture healing and are thus a critical step for influencing survival and function of immune cells in the early fracture hematoma.
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