ArticleNucleic acids research2013
Study of PcaV from Streptomyces coelicolor yields new insights into ligand-responsive MarR family transcription factors.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 34 papers.
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Who cites it
34 citing papers in PubMed, 67 citations in OpenAlex.
- A conservedFrontiers in microbiology · 2026Article
- Exploring plant-derived phytochrome chaperone proteins for light-switchable transcriptional regulation in mammals.Nature communications · 2024Article
- Review
- MarR Family Transcriptional Regulators and Their Roles in Plant-Interacting Bacteria.Microorganisms · 2023Review
- PicR as a MarR Family Transcriptional Repressor Multiply Controls the Transcription of Picolinic Acid Degradation Gene ClusterApplied and environmental microbiology · 2022Article
- Directed Evolution of Transcription Factor-Based Biosensors for Altered Effector Specificity.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Formaldehyde-responsive proteins, TtmR and EfgA, reveal a tradeoff between formaldehyde resistance and efficient transition to methylotrophy inJournal of bacteriology · 2021Article
- The MarR-Type Regulator PA3458 Is Involved in Osmoadaptation Control inInternational journal of molecular sciences · 2021Article
- Uncovering and Engineering a Mini-Regulatory Network of the TetR-Family Regulator SACE_0303 for Yield Improvement of Erythromycin inFrontiers in bioengineering and biotechnology · 2021Article
- Specificity of protein-DNA interactions in hypersaline environment: structural studies on complexes of Halobacterium salinarum oxidative stress-dependent protein hsRosR.Nucleic acids research · 2019Article
- LmbU, a Cluster-Situated Regulator for Lincomycin, Consists of a DNA-Binding Domain, an Auto-Inhibitory Domain, and Forms Homodimer.Frontiers in microbiology · 2019Article
- Directed evolution of the PcaV allosteric transcription factor to generate a biosensor for aromatic aldehydes.Journal of biological engineering · 2019Article
- Structural basis of transcriptional regulation by CouR, a repressor of coumarate catabolism, inThe Journal of biological chemistry · 2018Article
- A peculiar IclR family transcription factor regulates para-hydroxybenzoate catabolism in Streptomyces coelicolor.Nucleic acids research · 2018Article
- The Novel Transcriptional Regulator LmbU Promotes Lincomycin Biosynthesis through Regulating Expression of Its Target Genes in Streptomyces lincolnensis.Journal of bacteriology · 2018Article
- Mechanistic studies of DepR in regulating FK228 biosynthesis in Chromobacterium violaceum no. 968.PloS one · 2018Article
- Functional Mechanism of the Efflux Pumps Transcription Regulators FromFrontiers in molecular biosciences · 2018Review
- SAV4189, a MarR-Family Regulator inFrontiers in microbiology · 2018Article
- Isolation and Transcriptome Analysis of Phenol-Degrading Bacterium From Carbon-Sand Filters in a Full-Scale Drinking Water Treatment Plant.Frontiers in microbiology · 2018Article
- Structural basis of effector and operator recognition by the phenolic acid-responsive transcriptional regulator PadR.Nucleic acids research · 2017Article
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4 authors at 1 institution in 1 country.
Funding
Abstract
MarR family proteins constitute a group of >12 000 transcriptional regulators encoded in bacterial and archaeal genomes that control gene expression in metabolism, stress responses, virulence and multi-drug resistance. There is much interest in defining the molecular mechanism by which ligand binding attenuates the DNA-binding activities of these proteins. Here, we describe how PcaV, a MarR family regulator in Streptomyces coelicolor, controls transcription of genes encoding β-ketoadipate pathway enzymes through its interaction with the pathway substrate, protocatechuate. This transcriptional repressor is the only MarR protein known to regulate this essential pathway for aromatic catabolism. In in vitro assays, protocatechuate and other phenolic compounds disrupt the PcaV-DNA complex. We show that PcaV binds protocatechuate in a 1:1 stoichiometry with the highest affinity of any MarR family member. Moreover, we report structures of PcaV in its apo form and in complex with protocatechuate. We identify an arginine residue that is critical for ligand coordination and demonstrate that it is also required for binding DNA. We propose that interaction of ligand with this arginine residue dictates conformational changes that modulate DNA binding. Our results provide new insights into the molecular mechanism by which ligands attenuate DNA binding in this large family of transcription factors.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.