ArticleNature biotechnology2013
Synthetic circuits integrating logic and memory in living cells.
Article in Nature biotechnology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 231 papers.
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Who cites it
231 citing papers in PubMed.
- A decoupled transcription platform enables tunable and predictable gene expression in yeast.Nature communications · 2026Article
- Deep Learning Predicts Dissimilar DNA-DNA Binding and Engineers Hyperconnected Networks.Nature communications · 2026Article
- Cascading recombinase memory switch for programmable and stable gene expression in Pseudomonas putida.Nucleic acids research · 2026Article
- Construction of synthetic protein-binding non-genetic DNA systems in living cells.Nature chemistry · 2026Article
- Bacterial gene regulatory neural network as a biocomputing library of mathematical solvers.NPJ systems biology and applications · 2026Article
- Synthetic circuits for cell ratio control.Nature · 2026Article
- Tunable low-rate genomic recombination with Cre-Applied and environmental microbiology · 2026Article
- Dynamics of genetic circuits in Pseudomonas protegens.Cell systems · 2026Article
- Gene-sized editing for the therapy of genetic diseases.Functional & integrative genomics · 2026Review
- SUPER: Upcycling Genetic Parts for Precise Gene Expression Control, Leakage Minimization, and Genetic Circuit Stability.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- Design of Conditional Guide RNAs for the Logical Regulation of Gene Expression.Methods in molecular biology (Clifton, N.J.) · 2026Article
- Base gap switches as transcriptional regulators and their applications as biosensors.Nature sensors · 2026Article
- Programmable multistep CRISPR gene activation via control of RNA polymerase III termination.Science advances · 2025Article
- "AND" or "OR" logic operations of DNA probes: Fluorescent detection and discrimination of ovarian cancer cells via dual-microRNA in complex environments.Materials today. Bio · 2025Article
- MitoScribe single-cell molecular recorder logs graded signaling dynamics into mitochondrial DNA.bioRxiv : the preprint server for biology · 2025Article
- Gene circuit-based sensors.Fundamental research · 2025Review
- Characterization of recombinase activity across cellular growth phases.Scientific reports · 2025Article
- Expanding the σ54-dependent transcription process with orthogonal designs.Nucleic acids research · 2025Article
- Quantum-inspired logic for advanced Transcriptional Programming.Nucleic acids research · 2025Article
171 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Logic and memory are essential functions of circuits that generate complex, state-dependent responses. Here we describe a strategy for efficiently assembling synthetic genetic circuits that use recombinases to implement Boolean logic functions with stable DNA-encoded memory of events. Application of this strategy allowed us to create all 16 two-input Boolean logic functions in living Escherichia coli cells without requiring cascades comprising multiple logic gates. We demonstrate long-term maintenance of memory for at least 90 cell generations and the ability to interrogate the states of these synthetic devices with fluorescent reporters and PCR. Using this approach we created two-bit digital-to-analog converters, which should be useful in biotechnology applications for encoding multiple stable gene expression outputs using transient inputs of inducers. We envision that this integrated logic and memory system will enable the implementation of complex cellular state machines, behaviors and pathways for therapeutic, diagnostic and basic science applications.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.