ArticleJournal of the American Chemical Society2013
Chiral sum frequency generation for in situ probing proton exchange in antiparallel β-sheets at interfaces.
Article in Journal of the American Chemical Society, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Distinct adsorption behavior and structures of cell-penetrating peptides at a model lipid membrane interface: a heterodyne-detected vibrational sum frequency generation spectroscopy study.Chemical science · 2026Article
- Probing the structure of water in individual living cells.Nature communications · 2024Article
- Ultrafast control over chiral sum-frequency generation.Science advances · 2023Article
- Detecting Interplay of Chirality, Water, and Interfaces for Elucidating Biological Functions.Accounts of chemical research · 2023Article
- Intrinsically Disordered Osteopontin Fragment Orders During Interfacial Calcium Oxalate Mineralization.Angewandte Chemie (International ed. in English) · 2021Article
- Mirror-image antiparallel β-sheets organize water molecules into superstructures of opposite chirality.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Chiral Inversion of Amino Acids in Antiparallel β-Sheets at Interfaces Probed by Vibrational Sum Frequency Generation Spectroscopy.The journal of physical chemistry. B · 2019Article
- Chiral Water Superstructures around Antiparallel β-Sheets Observed by Chiral Vibrational Sum Frequency Generation Spectroscopy.The journal of physical chemistry letters · 2019Article
- Hygroscopic compounds in spider aggregate glue remove interfacial water to maintain adhesion in humid conditions.Nature communications · 2018Article
- Observation of pH-Induced Protein Reorientation at the Water Surface.The journal of physical chemistry letters · 2017Article
- A narrow amide I vibrational band observed by sum frequency generation spectroscopy reveals highly ordered structures of a biofilm protein at the air/water interface.Chemical communications (Cambridge, England) · 2016Article
- New Insights from Sum Frequency Generation Vibrational Spectroscopy into the Interactions of Islet Amyloid Polypeptides with Lipid Membranes.Journal of diabetes research · 2016Review
- Broad-Bandwidth Chiral Sum Frequency Generation Spectroscopy for Probing the Kinetics of Proteins at Interfaces.Langmuir : the ACS journal of surfaces and colloids · 2015Article
- Structural Characterization of Single-Stranded DNA Monolayers Using Two-Dimensional Sum Frequency Generation Spectroscopy.The journal of physical chemistry. B · 2015Article
- Theoretical Sum Frequency Generation Spectroscopy of Peptides.The journal of physical chemistry. B · 2015Article
- Characterization of Parallel β-Sheets at Interfaces by Chiral Sum Frequency Generation Spectroscopy.The journal of physical chemistry letters · 2015Article
- Two-dimensional sum-frequency generation reveals structure and dynamics of a surface-bound peptide.Journal of the American Chemical Society · 2014Article
- Site-specific orientation of an α-helical peptide ovispirin-1 from isotope-labeled SFG spectroscopy.The journal of physical chemistry. B · 2013Article
- Lipid Fluid-Gel Phase Transition Induced Alamethicin Orientational Change Probed by Sum Frequency Generation Vibrational Spectroscopy.The journal of physical chemistry. C, Nanomaterials and interfaces · 2013Article
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Authors and funding
5 authors.
Funding
Abstract
Studying hydrogen/deuterium (H/D) exchange in proteins can provide valuable insight on protein structure and dynamics. Several techniques are available for probing H/D exchange in the bulk solution, including NMR, mass spectroscopy, and Fourier transform infrared spectroscopy. However, probing H/D exchange at interfaces is challenging because it requires surface-selective methods. Here, we introduce the combination of in situ chiral sum frequency generation (cSFG) spectroscopy and ab initio simulations of cSFG spectra as a powerful methodology to probe the dynamics of H/D exchange at interfaces. This method is applied to characterize H/D exchange in the antiparallel β-sheet peptide LK7β. We report here for the first time that the rate of D-to-H exchange is about 1 order of magnitude faster than H-to-D exchange in the antiparallel structure at the air/water interface, which is consistent with the existing knowledge that O-H/D dissociation in water is the rate-limiting step, and breaking the O-D bond is slower than breaking the O-H bond. The reported analysis also provides fundamental understanding of several vibrational modes and their couplings in peptide backbones that have been difficult to characterize by conventional methods, including Fermi resonances of various combinations of peptide vibrational modes such as amide I and amide II, C-N stretch, and N-H/N-D bending. These results demonstrate cSFG as a sensitive technique for probing the kinetics of H/D exchange in proteins at interfaces, with high signal-to-noise N-H/N-D stretch bands that are free of background from the water O-H/O-D stretch.
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