ArticleBMC cancer2013
Multicellular tumor spheroid models to explore cell cycle checkpoints in 3D.
Article in BMC cancer, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed.
- Adaptation of Non-Invasive Cancer Cells to 3D Collagen I Microenvironment Induces Transcriptional Reprogramming Accompanied by a More Complex RNA Landscape.International journal of molecular sciences · 2026Article
- FOXO1-mediated cell cycle arrest enhances osteogenic differentiation of spheroid-cultured human periodontal ligament-derived multipotent mesenchymal stromal cells.Regenerative therapy · 2026Article
- Metabolic starvation-induced cell swelling drives solid stress in tumors.bioRxiv : the preprint server for biology · 2026Article
- Integrated light and electron microscopy workflow for morphological, molecular and ultrastructural analysis of spheroids.PloS one · 2026Article
- Aquaporin 5 expression regulates MDA-MB-231 spheroid multicellular invasion.American journal of physiology. Cell physiology · 2025Article
- The 3D Language of Cancer: Communication via Extracellular Vesicles from Tumor Spheroids and Organoids.International journal of molecular sciences · 2025Review
- Review
- Spatiotemporal analysis of ratiometric biosensors in live multicellular spheroids using SPoRTS.Cell reports methods · 2025Article
- Fibril-Guided Three-Dimensional Assembly of Human Fibroblastic Reticular Cells.ACS applied bio materials · 2024Article
- The enhanced energy metabolism in the tumor margin mediated by RRAD promotes the progression of oral squamous cell carcinoma.Cell death & disease · 2024Article
- Scaffold-free development of multicellular tumor spheroids with spatial characterization of structure and metabolic radial profiles.In vitro models · 2024Article
- Size-Specific Modulation of a Multienzyme Glucosome Assembly during the Cell Cycle.ACS bio & med chem Au · 2023Article
- PKM2 dictates the poised chromatin state ofNAR cancer · 2023Article
- Article
- Macrophage induces anti-cancer drug resistance in canine mammary gland tumor spheroid.Scientific reports · 2023Article
- GelMA and Biomimetic Culture Allow the Engineering of Mineralized, Adipose, and Tumor Tissue Human Microenvironments for the Study of Advanced Prostate Cancer In Vitro and In Vivo.Advanced healthcare materials · 2023Article
- Design and Study of a Reflector-Separated Light Dispersion-Compensated 3D Microscopy System.Sensors (Basel, Switzerland) · 2023Article
- Applications and Advances of Multicellular Tumor Spheroids: Challenges in Their Development and Analysis.International journal of molecular sciences · 2023Review
- State-dependent evolutionary models reveal modes of solid tumour growth.Nature ecology & evolution · 2023Article
- Changes of protein expression during tumorosphere formation of small cell lung cancer circulating tumor cells.Oncology research · 2023Article
1 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
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Abstract
backgroundMultiCellular Tumor Spheroid (MCTS) mimics the organization of a tumor and is considered as an invaluable model to study cancer cell biology and to evaluate new antiproliferative drugs. Here we report how the characteristics of MCTS in association with new technological developments can be used to explore the regionalization and the activation of cell cycle checkpoints in 3D.
methodsCell cycle and proliferation parameters were investigated in Capan-2 spheroids by immunofluorescence staining, EdU incorporation and using cells engineered to express Fucci-red and -green reporters.
resultsWe describe in details the changes in proliferation and cell cycle parameters during spheroid growth and regionalization. We report the kinetics and regionalized aspects of cell cycle arrest in response to checkpoint activation induced by EGF starvation, lovastatin treatment and etoposide-induced DNA damage.
conclusionOur data present the power and the limitation of spheroids made of genetically modified cells to explore cell cycle checkpoints. This study paves the way for the investigation of molecular aspects and dynamic studies of the response to novel antiproliferative agents in 3D models.
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