ArticleJournal of agricultural and food chemistry2013
Phage-displayed peptide that mimics aflatoxins and its application in immunoassay.
Article in Journal of agricultural and food chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 20 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
20 citing papers in PubMed, 62 citations in OpenAlex.
- Specific Binding Agents for the Molecular Recognition of Biotoxins: Recent Advances and Applications in Forensic Toxicology.Molecules (Basel, Switzerland) · 2026Review
- Biotin-labelled peptidomimetic for competitive time-resolved fluoroimmunoassay of benzothiostrobin.Analytical and bioanalytical chemistry · 2022Article
- Recent Progress in Rapid Determination of Mycotoxins Based on Emerging Biorecognition Molecules: A Review.Toxins · 2022Review
- Mimotopes for Mycotoxins Diagnosis Based on Random Peptides or Recombinant Antibodies from Phage Library.Molecules (Basel, Switzerland) · 2021Review
- Bioluminescent detection of zearalenone using recombinant peptidomimetic Gaussia luciferase fusion protein.Mikrochimica acta · 2020Article
- Medicinal Plant Polyphenols Attenuate Oxidative Stress and Improve Inflammatory and Vasoactive Markers in Cerebral Endothelial Cells during Hyperglycemic Condition.Antioxidants (Basel, Switzerland) · 2020Article
- Assessment of the Microbiome and Potential Aflatoxin Associated With the Medicinal HerbFrontiers in microbiology · 2020Article
- Development of an Anti-Idiotypic VHH Antibody and Toxin-Free Enzyme Immunoassay for Ochratoxin A in Cereals.Toxins · 2019Article
- Development of a Nanobody-AviTag Fusion Protein and Its Application in a Streptavidin-Biotin-Amplified Enzyme-Linked Immunosorbent Assay for Ochratoxin A in Cereal.Analytical chemistry · 2018Article
- Nanobody Technology for Mycotoxin Detection in the Field of Food Safety: Current Status and Prospects.Toxins · 2018Review
- A peptide mimic blocks the cross-reaction of anti-DNA antibodies with glomerular antigens.Clinical and experimental immunology · 2016Article
- Multiple Bacteriophage Selection Strategies for Improved Affinity of a Peptide Targeting ERBB2.International journal of peptide research and therapeutics · 2015Article
- Competitive and noncompetitive phage immunoassays for the determination of benzothiostrobin.Analytica chimica acta · 2015Article
- Development of a nanobody-alkaline phosphatase fusion protein and its application in a highly sensitive direct competitive fluorescence enzyme immunoassay for detection of ochratoxin A in cereal.Analytical chemistry · 2015Article
- Enhanced sensitive immunoassay: noncompetitive phage anti-immune complex assay for the determination of malachite green and leucomalachite green.Journal of agricultural and food chemistry · 2014Article
- Development of phage immuno-loop-mediated isothermal amplification assays for organophosphorus pesticides in agro-products.Analytical chemistry · 2014Article
- Development of a heterologous enzyme-linked immunosorbent assay for organophosphorus pesticides with phage-borne peptide.RSC advances · 2014Article
- Selection of a mimotope peptide of S-adenosyl-L-homocysteine and its application in immunoassays.Molecules (Basel, Switzerland) · 2013Article
- Isolation of alpaca anti-idiotypic heavy-chain single-domain antibody for the aflatoxin immunoassay.Analytical chemistry · 2013Article
- Fluorimetric immunoassay for multianalysis of aflatoxins.Journal of analytical methods in chemistry · 2013Article
Corrections and comments
- Erratum issued
Authors and funding
7 authors at 3 institutions in 2 countries.
Funding
Abstract
To search for an alternative to using protein conjugated aflatoxin as a coating antigen in aflatoxin detection by an ELISA method, a random-8-peptide library was constructed and used as a source of peptides that mimic aflatoxins (termed as mimotopes). Five mimotope peptides were obtained by panning-elution from the library and were successfully used in an indirect competitive ELISA for analyzing total aflatoxin concentration. The assay exhibited an IC50 value of 14 μg/kg in samples (with 1 in 7 dilution of sample extract) for aflatoxins. The linear range is 4-24 μg/kg. Further validation indicated relatively good recovery (60-120%) in peanut, rice and corn. Natural contaminated samples (peanut and feedstuff) were analyzed for aflatoxin concentration by both conventional ELISA and phage ELISA. The results showed good correlation. It can be concluded that the mimotope preparation is an effective substitute for the aflatoxin based coating antigen in ELISA and can be used in real sample analysis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.