ArticleArteriosclerosis, thrombosis, and vascular biology2013
Thioredoxin-interacting protein mediates sustained VEGFR2 signaling in endothelial cells required for angiogenesis.
Article in Arteriosclerosis, thrombosis, and vascular biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 36 citations in OpenAlex.
- The protective role of endothelial GLUT1 in ischemic stroke.Brain and behavior · 2024Review
- Novel Mechanism by Which Extracellular Cyclic GMP Induces Natriuresis.Circulation research · 2023Article
- Insights into the Steps of Breast Cancer-Brain Metastases Development: Tumor Cell Interactions with the Blood-Brain Barrier.International journal of molecular sciences · 2022Article
- Cytoskeletal Remodeling Mimics Endothelial Response to Microgravity.Frontiers in cell and developmental biology · 2021Article
- 1‑Palmitoyl‑2‑linoleoyl‑3‑acetyl‑rac‑glycerol ameliorates EGF‑induced MMP‑9 expression by promoting receptor desensitization in MDA‑MB‑231 cells.Oncology reports · 2020Article
- Redox RegulationAntioxidants & redox signaling · 2020Review
- Sodium Butyrate Inhibits Neovascularization Partially via TNXIP/VEGFR2 Pathway.Oxidative medicine and cellular longevity · 2020Article
- Activating CD137 Signaling Promotes Sprouting Angiogenesis via Increased VEGFA Secretion and the VEGFR2/Akt/eNOS Pathway.Mediators of inflammation · 2020Article
- Thioredoxin-Interacting Protein (TXNIP) in Cerebrovascular and Neurodegenerative Diseases: Regulation and Implication.Molecular neurobiology · 2018Review
- A function-blocking CD47 antibody modulates extracellular vesicle-mediated intercellular signaling between breast carcinoma cells and endothelial cells.Journal of cell communication and signaling · 2018Article
- Nutrient supply affects the mRNA expression profile of the porcine skeletal muscle.BMC genomics · 2017Article
- Deletion of TXNIP Mitigates High-Fat Diet-Impaired Angiogenesis and Prevents Inflammation in a Mouse Model of Critical Limb Ischemia.Antioxidants (Basel, Switzerland) · 2017Article
- Dynasore impairs VEGFR2 signalling in an endocytosis-independent manner.Scientific reports · 2017Article
- Constitutive Endocytosis of VEGFR2 Protects the Receptor against Shedding.The Journal of biological chemistry · 2016Article
- Consequences of a Maternal High-Fat Diet and Late Gestation Diabetes on the Developing Rat Lung.PloS one · 2016Article
- Astrocytic TYMP and VEGFA drive blood-brain barrier opening in inflammatory central nervous system lesions.Brain : a journal of neurology · 2015Article
- Role of inflammasome activation in the pathophysiology of vascular diseases of the neurovascular unit.Antioxidants & redox signaling · 2015Review
- Regulation of β-adrenergic receptor trafficking and lung microvascular endothelial cell permeability by Rab5 GTPase.International journal of biological sciences · 2015Article
- Thioredoxin-interacting protein is a biomechanical regulator of Src activity: key role in endothelial cell stress fiber formation.Circulation research · 2014Article
- A critical role for thioredoxin-interacting protein in diabetes-related impairment of angiogenesis.Diabetes · 2014Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
objectiveThioredoxin-interacting protein (TXNIP) is an α-arrestin protein whose function is important for the regulation of vascular endothelial growth factor receptor 2 (VEGFR2) signaling and endothelial cell survival. Because VEGFR2 is critical for angiogenesis, we explored the role of TXNIP in VEGF-induced angiogenesis. APPROACH AND
resultsTXNIP knockdown inhibited VEGF-induced endothelial cell tube formation and proliferation in cultured human umbilical vein endothelial cell. To elucidate the mechanism by which TXNIP altered VEGFR2 signaling in human umbilical vein endothelial cell, we studied phosphorylation of VEGFR2, phospholipase C gamma-1 (PLCγ1), endothelial NO synthase, and Akt (known as protein kinase B). TXNIP knockdown significantly decreased phosphorylation of VEGFR2 and PLCγ1 at times >5 minutes, but phosphorylation was unchanged at 2 minutes, as was Akt and endothelial NO synthase phosphorylation. Cell-surface biotinylation assay showed that TXNIP knockdown significantly attenuated VEGFR2 internalization. These results suggested that TXNIP was required for sustained VEGFR2 signaling, which is mediated largely by internalized VEGFR2. Rab5 knockdown to inhibit the trafficking and fusion of early endosomes significantly blocked VEGF-induced VEGFR2 internalization and phosphorylation of VEGFR2 and PLCγ1. Immunofluorescence and coimmunoprecipitation showed that TXNIP was part of a complex that included Rab5 and VEGFR2. Finally, TXNIP knockdown prevented the association of VEGFR2 and Rab5.
conclusionsOur results show that TXNIP is essential for VEGFR2 internalization in Rab5 positive endosomes, which is required for endothelial cell growth and angiogenesis.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.