ArticleNucleic acids research2013
Exploring sRNA-mediated gene silencing mechanisms using artificial small RNAs derived from a natural RNA scaffold in Escherichia coli.
Article in Nucleic acids research, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 36 citations in OpenAlex.
- Post-transcriptional control of bacterial nitrogen metabolism by regulatory noncoding RNAs.World journal of microbiology & biotechnology · 2022Review
- Effect of periostin silencing on Runx2, RANKL and OPG expression in osteoblasts.Journal of orofacial orthopedics = Fortschritte der Kieferorthopadie : Organ/official journal Deutsche Gesellschaft fur Kieferorthopadie · 2021Article
- Orchestration of virulence factor expression and modulation of biofilm dispersal in Erwinia amylovora through activation of the Hfq-dependent small RNA RprA.Molecular plant pathology · 2021Article
- Synthetic small regulatory RNAs in microbial metabolic engineering.Applied microbiology and biotechnology · 2021Review
- Global mRNA and microRNA expression dynamics in response to anthracnose infection in sorghum.BMC genomics · 2020Article
- Mechanism for coordinate regulation ofRNA biology · 2020Article
- Coordinate regulation of the expression of SdsR toxin and its downstream pphA gene by RyeA antitoxin in Escherichia coli.Scientific reports · 2019Article
- Mechanisms for Hfq-Independent Activation ofMolecules and cells · 2019Article
- The small RNA, SdsR, acts as a novel type of toxin in Escherichia coli.RNA biology · 2018Article
- Balancing gene expression without library construction via a reusable sRNA pool.Nucleic acids research · 2017Article
- Visualized and precise design of artificial small RNAs for regulating T7 RNA polymerase and enhancing recombinant protein folding inSynthetic and systems biotechnology · 2016Article
- Identification of novel sRNAs involved in biofilm formation, motility, and fimbriae formation in Escherichia coli.Scientific reports · 2015Article
- Effects of different target sites on antisense RNA-mediated regulation of gene expression.BMB reports · 2014Article
- Large-scale mapping of sequence-function relations in small regulatory RNAs reveals plasticity and modularity.Nucleic acids research · 2014Article
- Small RNAs in mycobacteria: an unfolding story.Frontiers in cellular and infection microbiology · 2014Review
- Regulatory RNAs: charming gene management styles for synthetic biology applications.RNA biology · 2013Review
- (Non-)translational medicine: targeting bacterial RNA.Frontiers in genetics · 2013Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
An artificial small RNA (afsRNA) scaffold was designed from an Escherichia coli sRNA, SibC. Using the lacZ reporter system, the gene silencing effects of afsRNAs were examined to explore the sRNA-mediated gene-silencing mechanisms in E. coli. Substitution of the original target recognition sequence with a new sequence recognizing lacZ mRNA led to effective reduction of lacZ gene expression. Single-strandedness of the target recognition sequences in the scaffold was essential for effective gene silencing. The target recognition sequence was shortened to 10 nt without significant loss of gene silencing, although this minimal length was limited to a specific target mRNA sequence. In cases where afsRNAs had mismatched (forming internal loops) or unmatched (forming bulges) regions in the middle of the target recognition sequence, internal loop-forming afsRNAs were more effective in gene silencing than those that formed bulges. Unexpectedly, gene silencing by afsRNA was not decreased but increased on hfq disruption in E. coli, particularly when interactions between afsRNA and mRNA were weak, suggesting that Hfq is possibly involved in destabilization of the RNA-RNA duplex, rather than enhancement of base pairing.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.