ArticleGenes & development2013
Multiple roles for Piwi in silencing Drosophila transposons.
Article in Genes & development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 169 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
169 citing papers in PubMed, 264 citations in OpenAlex.
- PIWIL4 regulates gene expression and piRNA levels in RSV-infected airway epithelial cells.iScience · 2026Article
- Unraveling the role of PIWI/piRNAs in stem cell therapy: Epigenetic mechanisms and therapeutic potentials.Genes & diseases · 2026Review
- The piRNA pathway mediates transcriptional silencing of LTR retrotransposons in ovaries and somatic tissues of Aedes mosquitoes.Nucleic acids research · 2026Article
- The novel HP1a interactor Clump/CG30403 safeguards transposon silencing and reproductive resilience in thebioRxiv : the preprint server for biology · 2026Article
- Host-transposable element coexistence: a matter of resistance, tolerance and trade-off.The EMBO journal · 2026Review
- In thebioRxiv : the preprint server for biology · 2026Article
- piRNA direct the chromatin reader to its genomic targets.bioRxiv : the preprint server for biology · 2026Article
- AbioRxiv : the preprint server for biology · 2026Article
- PIWIL4 regulates gene expression and piRNA levels in RSV-infected airway epithelial cells.bioRxiv : the preprint server for biology · 2025Article
- RNA-binding protein Ars2 mediates transcriptional silencing of telomeric repeats and transposable elements in the Drosophila germline.Nucleic acids research · 2025Article
- Paramutation-Like Behavior of Genic piRNA-Producing Loci inInternational journal of molecular sciences · 2025Article
- Transcriptional coupling of telomeric retrotransposons with the cell cycle.Science advances · 2025Article
- PIWIL genes in hepatocellular carcinoma: a multi-omics approach uncovering dysregulated expression and ceRNA networks in mice.BMC genomic data · 2024Article
- The NSL complex is required for piRNA production from telomeric clusters.Life science alliance · 2023Article
- Pervasive SUMOylation of heterochromatin and piRNA pathway proteins.Cell genomics · 2023Article
- Modeling early germline immunization after horizontal transfer of transposable elements reveals internal piRNA cluster heterogeneity.BMC biology · 2023Article
- Repetitive elements in aging and neurodegeneration.Trends in genetics : TIG · 2023Review
- The emerging role of the piRNA/PIWI complex in respiratory tract diseases.Respiratory research · 2023Review
- Off-target piRNA gene silencing in Drosophila melanogaster rescued by a transposable element insertion.PLoS genetics · 2023Article
- Review
109 more citing papers are in PubMed but not listed here.
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Silencing of transposons in the Drosophila ovary relies on three Piwi family proteins--Piwi, Aubergine (Aub), and Ago3--acting in concert with their small RNA guides, the Piwi-interacting RNAs (piRNAs). Aub and Ago3 are found in the germ cell cytoplasm, where they function in the ping-pong cycle to consume transposon mRNAs. The nuclear Piwi protein is required for transposon silencing in both germ and somatic follicle cells, yet the precise mechanisms by which Piwi acts remain largely unclear. We investigated the role of Piwi by combining cell type-specific knockdowns with measurements of steady-state transposon mRNA levels, nascent RNA synthesis, chromatin state, and small RNA abundance. In somatic cells, Piwi loss led to concerted effects on nascent transcripts and transposon mRNAs, indicating that Piwi acts through transcriptional gene silencing (TGS). In germ cells, Piwi loss showed disproportionate impacts on steady-state RNA levels, indicating that it also exerts an effect on post-transcriptional gene silencing (PTGS). Piwi knockdown affected levels of germ cell piRNAs presumably bound to Aub and Ago3, perhaps explaining its post-transcriptional impacts. Overall, our results indicate that Piwi plays multiple roles in the piRNA pathway, in part enforcing transposon repression through effects on local chromatin states and transcription but also participating in germ cell piRNA biogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.