ArticleRNA biology2013
Comparative analysis ofCas6b processing and CRISPR RNA stability.
Article in RNA biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 11 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
11 citing papers in PubMed, 23 citations in OpenAlex.
- A type III-associated Cas6 functions as a negative regulator of type I-B CRISPR-Cas system in Thermus thermophilus.Communications biology · 2025Article
- Digging into the lesser-known aspects of CRISPR biology.International microbiology : the official journal of the Spanish Society for Microbiology · 2021Review
- Heavily Armed Ancestors: CRISPR Immunity and Applications in Archaea with a Comparative Analysis of CRISPR Types in Sulfolobales.Biomolecules · 2020Review
- Cross-cleavage activity of Cas6b in crRNA processing of two different CRISPR-Cas systems in Methanosarcina mazei Gö1.RNA biology · 2019Article
- A Non-Stem-Loop CRISPR RNA Is Processed by Dual Binding Cas6.Structure (London, England : 1993) · 2016Article
- Article
- Biogenesis pathways of RNA guides in archaeal and bacterial CRISPR-Cas adaptive immunity.FEMS microbiology reviews · 2015Review
- DNA and RNA interference mechanisms by CRISPR-Cas surveillance complexes.FEMS microbiology reviews · 2015Review
- Annotation and Classification of CRISPR-Cas Systems.Methods in molecular biology (Clifton, N.J.) · 2015Review
- Structure of a dimeric crenarchaeal Cas6 enzyme with an atypical active site for CRISPR RNA processing.The Biochemical journal · 2013Article
- Special focus CRISPR-Cas.RNA biology · 2013Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The prokaryotic antiviral defense systems CRISP R (clustered regularly interspaced short palindromic repeats)/Cas (CRISP Rassociated) employs short crRNAs (CRISP R RNAs) to target invading viral nucleic acids. A short spacer sequence of these crRNAs can be derived from a viral genome and recognizes a reoccurring attack of a virus via base complementarity. We analyzed the effect of spacer sequences on the maturation of crRNAs of the subtype I-B Methanococcus maripaludis C5 CRISP R cluster. The responsible endonuclease, termed Cas6b, bound non-hydrolyzable repeat RNA as a dimer and mature crRNA as a monomer. Comparative analysis of Cas6b processing of individual spacer-repeat-spacer RNA substrates and crRNA stability revealed the potential influence of spacer sequence and length on these parameters. Correlation of these observations with the variable abundance of crRNAs visualized by deep-sequencing analyses is discussed. Finally, insertion of spacer and repeat sequences with archaeal poly-T termination signals is suggested to be prevented in archaeal CRISP R/Cas systems.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.