ArticleThe Indian journal of medical research2012
Improvised microinjection technique for mosquito vectors.
Article in The Indian journal of medical research, 2012. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
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Who cites it
6 citing papers in PubMed.
- RNAi-Mediated Silencing ofInsects · 2024Article
- RNA interference-mediated knockdown of 3, 4-dihydroxyphenylacetaldehyde synthase affects larval development and adult survival in the mosquito Aedes aegypti.Parasites & vectors · 2019Article
- Use of a Recombinant Mosquito Densovirus As a Gene Delivery Vector for the Functional Analysis of Genes in Mosquito Larvae.Journal of visualized experiments : JoVE · 2017Article
- Wolbachia: The selfish Trojan Horse in dengue control.Medical journal, Armed Forces India · 2016Review
- Testing the causality between CYP9M10 and pyrethroid resistance using the TALEN and CRISPR/Cas9 technologies.Scientific reports · 2016Article
- Identification of differentially expressed microRNAs in Culex pipiens and their potential roles in pyrethroid resistance.Insect biochemistry and molecular biology · 2014Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BACKGROUND &
objectivesBio-manipulation technique is of primary importance during the development of transgenic mosquitoes. The study describes the variable factors that influence the viability of medically important mosquito vectors during microinjection.
methodsThree mosquito vectors belonging to the genus Aedes, Anopheles and Culex were microinjected at different developmental stages of their life cycle viz., egg, larvae, pupae and adult.
resultsThe improvisations revealed an increased survivability of biomanipulated mosquitoes during the embryonic and adult microinjection. The study of injecting larvae and pupae resulted in poor survivability. INTERPRETATION &
conclusionsThe microinjection protocol was successfully tested on three important mosquito vectors. The critical period after biomanipulation which contributes heavily for the survivability factor was evaluated. The results provide a common protocol for biomanipulation of three mosquito vectors with enhanced survivability.
Indexed as
Identifiers
23391792PMC3612326What OpenQuestion holds
Registered trials
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