ArticleAnalytical chemistry2013
Toward implementation of quorum sensing autoinducers as biomarkers for infectious disease states.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 38 citations in OpenAlex.
- Emerging advances in biosensor technologies for quorum sensing signal molecules.Analytical and bioanalytical chemistry · 2025Review
- Development of an analytical method to quantify N-acyl-homoserine lactones in bacterial cultures, river water, and treated wastewater.Analytical and bioanalytical chemistry · 2024Article
- Detection of gut microbiota and pathogen produced N-acyl homoserine in host circulation and tissues.NPJ biofilms and microbiomes · 2021Article
- Inhibitory effects of novel 1,4-disubstituted 1,2,3-triazole compounds on quorum-sensing of P. aeruginosa PAO1.European journal of clinical microbiology & infectious diseases : official publication of the European Society of Clinical Microbiology · 2021Article
- Targeted and untargeted quantification of quorum sensing signalling molecules in bacterial cultures and biological samples via HPLC-TQ MS techniques.Analytical and bioanalytical chemistry · 2021Article
- Detection of Quorum-Sensing Molecules for Pathogenic Molecules Using Cell-Based and Cell-Free Biosensors.Antibiotics (Basel, Switzerland) · 2020Review
- Vertebrate odorant binding proteins as antimicrobial humoral components of innate immunity for pathogenic microorganisms.PloS one · 2019Article
- Albumin Inhibits Pseudomonas aeruginosa Quorum Sensing and Alters Polymicrobial Interactions.Infection and immunity · 2017Article
- 5-Hydroxyethyl-3-tetradecanoyltetramic acid represents a novel treatment for intravascular catheter infections due to Staphylococcus aureus.The Journal of antimicrobial chemotherapy · 2017Article
- Peptides as Quorum Sensing Molecules: Measurement Techniques and Obtained LevelsFrontiers in neuroscience · 2017Review
- Innate Immune Signaling Activated by MDR Bacteria in the Airway.Physiological reviews · 2016Review
- Pseudomonas aeruginosa quorum sensing molecules correlate with clinical status in cystic fibrosis.The European respiratory journal · 2015Observational
- Novel Paraoxonase 2-Dependent Mechanism Mediating the Biological Effects of the Pseudomonas aeruginosa Quorum-Sensing Molecule N-(3-Oxo-Dodecanoyl)-L-Homoserine Lactone.Infection and immunity · 2015Article
- Impact of storage conditions on metabolite profiles of sputum samples from persons with cystic fibrosis.Journal of cystic fibrosis : official journal of the European Cystic Fibrosis Society · 2015Article
- Interkingdom signaling and its consequences for human health.Virulence · 2014Article
Corrections and comments
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Authors and funding
9 authors at 1 institution in 1 country.
Funding
Abstract
The opportunistic bacterial pathogen Pseudomonas aeruginosa causes chronic lung infections in cystic fibrosis (CF) patients. Importantly, virulence factor expression and biofilm formation in P. aeruginosa is coordinated by quorum sensing (QS) and one of the key QS signaling molecules is 3-oxo-C12-HSL. Remarkably, a tetramic acid, (C12-TA), with antibacterial properties is formed spontaneously from 3-oxo-C12-HSL under physiological conditions. Seeking to better understand this relationship, we sought to investigate whether 3-oxo-C12-HSL and C12-TA may be contributing factors to the overall pathogenicity of P. aeruginosa in CF individuals and if their detection and quantitation in sputum samples might be used as an indicator to assess disease states and monitor therapy success in CF patients. To this end, 3-oxo-C12-HSL and C12-TA concentrations were initially analyzed in P. aeruginosa flow cell biofilms using liquid chromatography coupled with mass spectrometry (LC-MS). A liquid chromatography tandem mass spectrometry (LC-MS/MS)-based method was then developed and validated for their detection and quantification in the sputa of CF patients. To the best of our knowledge, this is the first report to show the presence of both the quorum sensing molecule (3-oxo-C12-HSL) and its rearranged product (C12-TA) in human clinical samples such as sputum. A total of 47 sputum samples from 20 CF and 2 non-CF individuals were analyzed. 3-Oxo-C12-HSL was detected and quantified in 45 samples with concentrations ranging from 20 to >1000 nM; C12-TA was found in 14 samples (13-900 nM). On the basis of our findings, quorum sensing autoinducers merit further investigation as biomarkers for infectious disease states.
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Registered trials
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