ArticlePloS one2013
Phylogenetic analysis and DNA-based species confirmation in Anopheles (Nyssorhynchus).
Article in PloS one, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
45 citing papers in PubMed.
- An updated checklist of mosquitoes (Diptera, Culicidae) of Ecuador: new records and public health significance.ZooKeys · 2026Article
- First detection of Nyssorhynchus rondoniensis (Diptera: Culicidae) in southern Venezuela and its infection with Plasmodium falciparum.Acta tropica · 2025Article
- The toolbox for mosquito vector research.Parasites & vectors · 2025Review
- Rapid Specific PCR Detection Based onInternational journal of molecular sciences · 2025Article
- Molecular Identification ofMicroorganisms · 2025Article
- Distribution of Anophelinae (Diptera: Culicidae) and challenges for malaria elimination in Brazil.Memorias do Instituto Oswaldo Cruz · 2025Article
- DNA Barcoding for the Identification of Adult Mosquitoes (Diptera: Culicidae) in Western Australia.Ecology and evolution · 2024Article
- Characterization of larval habitats of Anopheles (Nyssorhynchus) darlingi and associated species in malaria areas in western Brazilian Amazon.Memorias do Instituto Oswaldo Cruz · 2024Article
- Genetic diversity and phylogeny ofHeliyon · 2023Article
- Phylogenetic relationships of the supercontig of sodium channel subunit I (NaV) in 17 species of Anopheles (Diptera: Culicidae).Revista da Sociedade Brasileira de Medicina Tropical · 2022Article
- Article
- Phylogenetic analysis of the Neotropical Albitarsis Complex based on mitogenome data.Parasites & vectors · 2021Article
- Complete mitogenomes of Anopheles peditaeniatus and Anopheles nitidus and phylogenetic relationships within the genus Anopheles inferred from mitogenomes.Parasites & vectors · 2021Article
- Newly Emerging Pest in China,Insects · 2021Article
- New Records of Anopheles benarrochi B (Diptera: Culicidae) in Malaria Hotspots in the Amazon Regions of Ecuador and Peru.Journal of medical entomology · 2021Article
- Identification keys to the Anopheles mosquitoes of South America (Diptera: Culicidae). IV. Adult females.Parasites & vectors · 2020Article
- Distribution and phylogenetic diversity of Anopheles species in malaria endemic areas of Honduras in an elimination setting.Parasites & vectors · 2020Article
- First record of translocation in Culicidae (Diptera) mitogenomes: evidence from the tribe Sabethini.BMC genomics · 2019Article
- Application of matrix-assisted laser desorption/ionization mass spectrometry to identify species of Neotropical Anopheles vectors of malaria.Malaria journal · 2019Article
- An insight into the sialotranscriptome and virome of Amazonian anophelines.BMC genomics · 2019Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Specimens of neotropical Anopheles (Nyssorhynchus) were collected and identified morphologically. We amplified three genes for phylogenetic analysis-the single copy nuclear white and CAD genes, and the COI barcode region. Since we had multiple specimens for most species we were able to test how well the single or combined genes were able to corroborate morphologically defined species by placing the species into exclusive groups. We found that single genes, including the COI barcode region, were poor at confirming species, but that the three genes combined were able to do so much better. This has implications for species identification, species delimitation, and species discovery, and we caution that single genes are not enough. Higher level groupings were partially resolved with some well-supported groupings, whereas others were found to be either polyphyletic or paraphyletic. There were examples of known groups, such as the Myzorhynchella Section, which were poorly supported with single genes but were well supported with combined genes. From this we can infer that more sequence data will be needed in order to show more higher-level groupings with good support. We got unambiguously good support (0.94-1.0 Bayesian posterior probability) from all DNA-based analyses for a grouping of An. dunhami with An. nuneztovari and An. goeldii, and because of this and because of morphological similarities we propose that An. dunhami be included in the Nuneztovari Complex. We obtained phylogenetic corroboration for new species which had been recognised by morphological differences; these will need to be formally described and named.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.