ArticleJournal of clinical microbiology2013
Validation of Mycobacterium tuberculosis Rv1681 protein as a diagnostic marker of active pulmonary tuberculosis.
Article in Journal of clinical microbiology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed, 30 citations in OpenAlex.
- Trial
- Exploring the Potential of Exosomes as Biomarkers in Tuberculosis and Other Diseases.International journal of molecular sciences · 2024Review
- Article
- Proteomic Analysis of Urine from Patients withTropical medicine and infectious disease · 2022Article
- Review of Liquid Chromatography-Mass Spectrometry-Based Proteomic Analyses of Body Fluids to Diagnose Infectious Diseases.International journal of molecular sciences · 2022Review
- Urine-Based Antigen (Protein) Detection Test for the Diagnosis of Visceral Leishmaniasis.Microorganisms · 2020Review
- Use of VHH antibodies for the development of antigen detection test for visceral leishmaniasis.Parasite immunology · 2018Article
- Discovery of a unique Mycobacterium tuberculosis protein through proteomic analysis of urine from patients with active tuberculosis.Microbes and infection · 2018Article
- Recent Trends in System-Scale Integrative Approaches for Discovering Protective Antigens Against Mycobacterial Pathogens.Frontiers in genetics · 2018Review
- Potential of High-Affinity, Slow Off-Rate Modified Aptamer Reagents for Mycobacterium tuberculosis Proteins as Tools for Infection Models and Diagnostic Applications.Journal of clinical microbiology · 2017Article
- Identification potential biomarkers in pulmonary tuberculosis and latent infection based on bioinformatics analysis.BMC infectious diseases · 2016Article
- Rapid Diagnosis of Tuberculosis from Analysis of Urine Volatile Organic Compounds.ACS sensors · 2016Article
- Identification of Mycobacterial Antigens in Human Urine by Use of Immunoglobulin G Isolated from Sera of Patients with Active Pulmonary Tuberculosis.Journal of clinical microbiology · 2016Article
- Detection of inflammatory biomarkers in saliva and urine: Potential in diagnosis, prevention, and treatment for chronic diseases.Experimental biology and medicine (Maywood, N.J.) · 2016Review
- Mannosylated lipoarabinomannan in serum as a biomarker candidate for subclinical bovine tuberculosis.BMC research notes · 2014Article
- Circulating Mycobacterium bovis peptides and host response proteins as biomarkers for unambiguous detection of subclinical infection.Journal of clinical microbiology · 2014Article
- Pathogen-derived biomarkers for active tuberculosis diagnosis.Frontiers in microbiology · 2014Review
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Authors and funding
12 authors at 6 institutions in 5 countries.
Funding
Abstract
The development of an accurate antigen detection assay for the diagnosis of active tuberculosis (TB) would represent a major clinical advance. Here, we demonstrate that the Mycobacterium tuberculosis Rv1681 protein is a biomarker for active TB with potential diagnostic utility. We initially identified, by mass spectroscopy, peptides from the Rv1681 protein in urine specimens from 4 patients with untreated active TB. Rabbit IgG anti-recombinant Rv1681 detected Rv1681 protein in lysates and culture filtrates of M. tuberculosis and immunoprecipitated it from pooled urine specimens from two TB patients. An enzyme-linked immunosorbent assay formatted with these antibodies detected Rv1681 protein in unconcentrated urine specimens from 11/25 (44%) TB patients and 1/21 (4.8%) subjects in whom TB was initially clinically suspected but then ruled out by conventional methods. Rv1681 protein was not detected in urine specimens from 10 subjects with Escherichia coli-positive urine cultures, 26 subjects with confirmed non-TB tropical diseases (11 with schistosomiasis, 5 with Chagas' disease, and 10 with cutaneous leishmaniasis), and 14 healthy subjects. These results provide strong validation of Rv1681 protein as a promising biomarker for TB diagnosis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.