ArticleJournal of biomedical optics2013
Confocal microscopy with strip mosaicing for rapid imaging over large areas of excised tissue.
Article in Journal of biomedical optics, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 48 papers.
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The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
48 citing papers in PubMed.
- High-Resolution Rapid Diagnostic Imaging of Whole Prostate Biopsies Using Video-Rate Fluorescence Structured Illumination Microscopy.Cancer research · 2015Trial
- Real-time Imaging of the Resection Bed Using a Handheld Probe to Reduce Incidence of Microscopic Positive Margins in Cancer Surgery.Cancer research · 2015Trial
- Strip-Scanning for Efficient Widefield Retinal OCTA Mosaicking.Translational vision science & technology · 2026Article
- Article
- Vacuum-based specimen holder for rapid surgical specimen imaging.Biomedical optics express · 2025Article
- Motion-tolerant 3D volumetric multimodality microscopy imaging of human skin with subcellular resolution and extended field-of-view.Communications biology · 2025Article
- Single color digital H&E staining with In-and-Out Net.Computerized medical imaging and graphics : the official journal of the Computerized Medical Imaging Society · 2024Article
- Piston-based specimen holder for rapid surgical and biopsy specimen imaging.Biomedical optics express · 2024Article
- An end-to-end workflow for nondestructive 3D pathology.Nature protocols · 2024Review
- An end-to-end workflow for non-destructive 3D pathology.bioRxiv : the preprint server for biology · 2023Article
- Comprehensive Surface Histology of Fresh Resection Margins With Rapid Open-Top Light-Sheet (OTLS) Microscopy.IEEE transactions on bio-medical engineering · 2023Article
- Nondestructive 3D Pathology with Light-Sheet Fluorescence Microscopy for Translational Research and Clinical Assays.Annual review of analytical chemistry (Palo Alto, Calif.) · 2023Review
- Article
- Constructing an In Vitro and In Vivo Flow Cytometry by Fast Line Scanning of Confocal Microscopy.Sensors (Basel, Switzerland) · 2023Article
- Rapid On-Site Microscopy and Mapping of Diagnostic Biopsies for See-And-Treat Guidance of Localized Prostate Cancer Therapy.Cancers · 2023Article
- High-speed light-sheet microscopy for the in-situ acquisition of volumetric histological images of living tissue.Nature biomedical engineering · 2022Article
- High-speed mosaic imaging using scanner-synchronized stage position sampling.Journal of biomedical optics · 2022Article
- Improved microscopy with ultraviolet surface excitation (MUSE) using high-index immersion illumination.Biomedical optics express · 2021Article
- Differentiation of breast tissue types for surgical margin assessment using machine learning and polarization-sensitive optical coherence tomography.Biomedical optics express · 2021Article
- Harnessing non-destructive 3D pathology.Nature biomedical engineering · 2021Review
Corrections and comments
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Authors and funding
7 authors.
Funding
Abstract
Confocal mosaicing microscopy is a developing technology platform for imaging tumor margins directly in freshly excised tissue, without the processing required for conventional pathology. Previously, mosaicing on 12-×-12 mm² of excised skin tissue from Mohs surgery and detection of basal cell carcinoma margins was demonstrated in 9 min. Last year, we reported the feasibility of a faster approach called "strip mosaicing," which was demonstrated on a 10-×-10 mm² of tissue in 3 min. Here we describe further advances in instrumentation, software, and speed. A mechanism was also developed to flatten tissue in order to enable consistent and repeatable acquisition of images over large areas. We demonstrate mosaicing on 10-×-10 mm² of skin tissue with 1-μm lateral resolution in 90 s. A 2.5-×-3.5 cm² piece of breast tissue was scanned with 0.8-μm lateral resolution in 13 min. Rapid mosaicing of confocal images on large areas of fresh tissue potentially offers a means to perform pathology at the bedside. Imaging of tumor margins with strip mosaicing confocal microscopy may serve as an adjunct to conventional (frozen or fixed) pathology for guiding surgery.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.