ArticleMolecular biology of the cell2013
Evidence for dynein and astral microtubule-mediated cortical release and transport of Gαi/LGN/NuMA complex in mitotic cells.
Article in Molecular biology of the cell, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed, 55 citations in OpenAlex.
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- Importin α/β promote Kif18B microtubule association and enhance microtubule destabilization activity.Molecular biology of the cell · 2023Article
- Annexin A1 is a polarity cue that directs mitotic spindle orientation during mammalian epithelial morphogenesis.Nature communications · 2023Article
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- Discovery of specific mutations in spinal muscular atrophy patients by next-generation sequencing.Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology · 2021Article
- Mechanochemical Crosstalk Produces Cell-Intrinsic Patterning of the Cortex to Orient the Mitotic Spindle.Current biology : CB · 2020Article
- Review
- Localization of GPSM2 in the Nucleus of Invasive Breast Cancer Cells Indicates a Poor Prognosis.Frontiers in oncology · 2020Article
- Cell shape and intercellular adhesion regulate mitotic spindle orientation.Molecular biology of the cell · 2019Article
- Proteostasis is essential during cochlear development for neuron survival and hair cell polarity.EMBO reports · 2019Article
- NDP52 tunes cortical actin interaction with astral microtubules for accurate spindle orientation.Cell research · 2019Article
- Eph signaling controls mitotic spindle orientation and cell proliferation in neuroepithelial cells.The Journal of cell biology · 2019Article
- The polarity-induced force imbalance inMolecular biology of the cell · 2018Article
- Insc:LGN tetramers promote asymmetric divisions of mammary stem cells.Nature communications · 2018Article
- The Light Intermediate Chain 2 Subpopulation of Dynein Regulates Mitotic Spindle Orientation.Scientific reports · 2016Article
- Nuclear Mitotic Apparatus (NuMA) Interacts with and Regulates Astrin at the Mitotic Spindle.The Journal of biological chemistry · 2016Article
- Regulation of mitotic spindle orientation: an integrated view.EMBO reports · 2016Review
- Coupling changes in cell shape to chromosome segregation.Nature reviews. Molecular cell biology · 2016Review
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Spindle positioning is believed to be governed by the interaction between astral microtubules and the cell cortex and involve cortically anchored motor protein dynein. How dynein is recruited to and regulated at the cell cortex to generate forces on astral microtubules is not clear. Here we show that mammalian homologue of Drosophila Pins (Partner of Inscuteable) (LGN), a Gαi-binding protein that is critical for spindle positioning in different systems, associates with cytoplasmic dynein heavy chain (DYNC1H1) in a Gαi-regulated manner. LGN is required for the mitotic cortical localization of DYNC1H1, which, in turn, also modulates the cortical accumulation of LGN. Using fluorescence recovery after photobleaching analysis, we show that cortical LGN is dynamic and the turnover of LGN relies, at least partially, on astral microtubules and DYNC1H1. We provide evidence for dynein- and astral microtubule-mediated transport of Gαi/LGN/nuclear mitotic apparatus (NuMA) complex from cell cortex to spindle poles and show that actin filaments counteract such transport by maintaining Gαi/LGN/NuMA and dynein at the cell cortex. Our results indicate that astral microtubules are required for establishing bipolar, symmetrical cortical LGN distribution during metaphase. We propose that regulated cortical release and transport of LGN complex along astral microtubules may contribute to spindle positioning in mammalian cells.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.