ArticleMarine drugs2013
Cloning, characterization and heterologous expression of the indolocarbazole biosynthetic gene cluster from marine-derived Streptomyces sanyensis FMA.
Article in Marine drugs, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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16 citing papers in PubMed.
- Morphology-engineered alleviation of mycelial aggregation inSynthetic and systems biotechnology · 2025Article
- Generation of streptocarbazoles with cytotoxicities by pathway engineering and insights into their biosynthesis.Applied and environmental microbiology · 2024Article
- Unusual Secondary Metabolites from the Mangrove Ecosystems: Structures, Bioactivities, Chemical, and Bio-Syntheses.Marine drugs · 2022Review
- Recent Advances in the Heterologous Expression of Biosynthetic Gene Clusters for Marine Natural Products.Marine drugs · 2022Review
- A Co-Culturing Approach Enables Discovery and Biosynthesis of a Bioactive Indole Alkaloid Metabolite.Molecules (Basel, Switzerland) · 2020Article
- Novel bioactive natural products from bacteria via bioprospecting, genome mining and metabolic engineering.Microbial biotechnology · 2019Review
- Staurosporine from Streptomyces sanyensis activates Programmed Cell Death in Acanthamoeba via the mitochondrial pathway and presents low in vitro cytotoxicity levels in a macrophage cell line.Scientific reports · 2019Article
- Overexpression of a type III PKS gene affording novel violapyrones with enhanced anti-influenza A virus activity.Microbial cell factories · 2018Article
- Cloning and Heterologous Expression of a Large-sized Natural Product Biosynthetic Gene Cluster inFrontiers in microbiology · 2017Review
- Article
- Generate a bioactive natural product library by mining bacterial cytochrome P450 patterns.Synthetic and systems biotechnology · 2016Review
- A Streptomyces coelicolor host for the heterologous expression of Type III polyketide synthase genes.Microbial cell factories · 2015Article
- Natural products from mangrove actinomycetes.Marine drugs · 2014Review
- Characterization of an environmental DNA-derived gene cluster that encodes the bisindolylmaleimide methylarcyriarubin.Chembiochem : a European journal of chemical biology · 2014Article
- Heterologous expression of natural product biosynthetic gene clusters in Streptomyces coelicolor: from genome mining to manipulation of biosynthetic pathways.Journal of industrial microbiology & biotechnology · 2014Review
- Modern Approaches to the Genome Editing of Antibiotic Biosynthetic Clusters in Actinomycetes.Acta naturaeArticle
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7 authors.
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Abstract
The indolocarbazole (ICZ) alkaloids have attracted much attention due to their unique structures and potential therapeutic applications. A series of ICZs were recently isolated and identified from a marine-derived actinomycete strain, Streptomyces sanyensis FMA. To elucidate the biosynthetic machinery associated with ICZs production in S. sanyensis FMA, PCR using degenerate primers was carried out to clone the FAD-dependent monooxygenase gene fragment for ICZ ring formation, which was used as a probe to isolate the 34.6-kb DNA region containing the spc gene cluster. Sequence analysis revealed genes for ICZ ring formation (spcO, D, P, C), sugar unit formation (spcA, B, E, K, J, I), glycosylation (spcN, G), methylation (spcMA, MB), as well as regulation (spcR). Their involvement in ICZ biosynthesis was confirmed by gene inactivation and heterologous expression in Streptomyces coelicolor M1152. This work represents the first cloning and characterization of an ICZ gene cluster isolated from a marine-derived actinomycete strain and would be helpful for thoroughly understanding the biosynthetic mechanism of ICZ glycosides.
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