ArticleInternational journal of molecular sciences2013
Preparation of DOPC and DPPC Supported Planar Lipid Bilayers for Atomic Force Microscopy and Atomic Force Spectroscopy.
Article in International journal of molecular sciences, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
73 citing papers in PubMed.
- Uniform Microscale Thermoresponsive Liposomes for Controlled Release Above Body Temperature.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Influence of Phospholipid Composition on Protein Adsorption to Lipid-Coated Silica Microparticles.Molecules (Basel, Switzerland) · 2026Article
- Molecular Interactions of Fluoroquinolone Antibiotics with Lipid Membranes.Langmuir : the ACS journal of surfaces and colloids · 2026Article
- Oxysterol Induced Fluid-Fluid Phase Separation in Saturated Phosphatidylcholine Membranes: Influence of Lipid Chain Length.The Journal of membrane biology · 2026Article
- Leveraging Viscosity to Unlock the Osteogenic Potential of BMP-2 Mimetic DWIVA.Small (Weinheim an der Bergstrasse, Germany) · 2026Article
- Effects of Lipopolysaccharides fromMembranes · 2026Article
- Design of a Trigger-Responsive Photothermal Vesicle-Based Cargo Delivery Platform.Small (Weinheim an der Bergstrasse, Germany) · 2025Article
- Monitoring Receptor Clustering by Aggregation-Induced Emission.ChemPlusChem · 2025Article
- Phospholipid membrane permeabilization and leakage of cell content by surfactin-CInternational microbiology : the official journal of the Spanish Society for Microbiology · 2025Article
- Septin higher-order structure on yeast membranes in vitro.Nature communications · 2025Article
- Leaflet-Specific Structure and Dynamics of Solid and Polymer Supported Lipid Bilayers.Angewandte Chemie (International ed. in English) · 2025Article
- SARS-CoV-2 FP1 Destabilizes Lipid Membranes and Facilitates Pore Formation.International journal of molecular sciences · 2025Article
- Nanostructural and nanomechanical alterations of photosensitized lipid membranes due to light induced formation of reactive oxygen species.Scientific reports · 2025Article
- All-Atom Simulations Reveal the Effect of Membrane Composition on the Signaling of the NKG2A/CD94/HLA-E Immune Receptor Complex.Journal of chemical information and modeling · 2024Article
- 2D Titanium Carbide MXene and Single-Molecule Fluorescence: Distance-Dependent Nonradiative Energy Transfer and Leaflet-Resolved Dye Sensing in Lipid Bilayers.Advanced materials (Deerfield Beach, Fla.) · 2024Article
- Exploring the Interaction of Lipid Bilayers with Curcumin-Laponite Nanoparticles: Implications for Drug Delivery and Therapeutic Applications.Small (Weinheim an der Bergstrasse, Germany) · 2024Article
- Mass and Stiffness Deconvolution in Nanomechanical Resonators for Precise Mass Measurement and In Vivo Biosensing.ACS nano · 2024Article
- Structure and thermodynamics of supported lipid membranes on hydrophobic van der Waals surfaces.Soft matter · 2024Article
- Review
- Atomic-scale structure of interfacial water on gel and liquid phase lipid membranes.Faraday discussions · 2024Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cell membranes are typically very complex, consisting of a multitude of different lipids and proteins. Supported lipid bilayers are widely used as model systems to study biological membranes. Atomic force microscopy and force spectroscopy techniques are nanoscale methods that are successfully used to study supported lipid bilayers. These methods, especially force spectroscopy, require the reliable preparation of supported lipid bilayers with extended coverage. The unreliability and a lack of a complete understanding of the vesicle fusion process though have held back progress in this promising field. We document here robust protocols for the formation of fluid phase DOPC and gel phase DPPC bilayers on mica. Insights into the most crucial experimental parameters and a comparison between DOPC and DPPC preparation are presented. Finally, we demonstrate force spectroscopy measurements on DOPC surfaces and measure rupture forces and bilayer depths that agree well with X-ray diffraction data. We also believe our approach to decomposing the force-distance curves into depth sub-components provides a more reliable method for characterising the depth of fluid phase lipid bilayers, particularly in comparison with typical image analysis approaches.
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.