ArticleOptics express2013
Single-molecule fluorescence imaging of processive myosin with enhanced background suppression using linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC).
Article in Optics express, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
20 citing papers in PubMed.
- Click chemistry-enabled gold nanorods for sensitive detection and viability evaluation of copper(II)-reducing bacteria.Materials today. Bio · 2025Article
- Strategies for Overcoming the Single-Molecule Concentration Barrier.ACS measurement science au · 2023Review
- Single-Molecule Mechanochemical Sensing.Accounts of chemical research · 2022Article
- Article
- Zero-mode waveguides and nanopore-based sequencing technologies accelerate single-molecule studies.Biophysics and physicobiology · 2022Article
- Single molecule turnover of fluorescent ATP by myosin and actomyosin unveil elusive enzymatic mechanisms.Communications biology · 2021Article
- A Single-Molecule Surface-Based Platform to Detect the Assembly and Function of the Human RNA Polymerase II Transcription Machinery.Structure (London, England : 1993) · 2020Article
- Real-time 3D single molecule tracking.Nature communications · 2020Article
- Real-Time 3D Single Particle Tracking: Towards Active Feedback Single Molecule Spectroscopy in Live Cells.Molecules (Basel, Switzerland) · 2019Review
- Miniaturized flow cell with pneumatically-actuated vertical nanoconfinement for single-molecule imaging and manipulation.Biomicrofluidics · 2018Article
- Zero-Mode Waveguide Nanophotonic Structures for Single Molecule Characterization.Journal of physics D: Applied physics · 2018Article
- Nanoscale devices for linkerless long-term single-molecule observation.Current opinion in biotechnology · 2016Review
- Single Filaments to Reveal the Multiple Flavors of Actin.Biophysical journal · 2016Review
- An improved surface passivation method for single-molecule studies.Nature methods · 2014Article
- Single-molecule tools for enzymology, structural biology, systems biology and nanotechnology: an update.Archives of toxicology · 2014Review
- High-throughput single-molecule studies of protein-DNA interactions.FEBS letters · 2014Review
- Adam Cohen: visualizing cellular voltage.The Journal of cell biology · 2014Article
- Photonic crystal enhancement of a homogeneous fluorescent assay using submicron fluid channels fabricated by E-jet patterning.Journal of biophotonics · 2014Article
- High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Use of fluorescent techniques to study the in vitro movement of myosins.Experientia supplementum (2012)Review
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Authors and funding
9 authors.
Funding
Abstract
Resolving single fluorescent molecules in the presence of high fluorophore concentrations remains a challenge in single-molecule biophysics that limits our understanding of weak molecular interactions. Total internal reflection fluorescence (TIRF) imaging, the workhorse of single-molecule fluorescence microscopy, enables experiments at concentrations up to about 100 nM, but many biological interactions have considerably weaker affinities, and thus require at least one species to be at micromolar or higher concentration. Current alternatives to TIRF often require three-dimensional confinement, and thus can be problematic for extended substrates, such as cytoskeletal filaments. To address this challenge, we have demonstrated and applied two new single-molecule fluorescence microscopy techniques, linear zero-mode waveguides (ZMWs) and convex lens induced confinement (CLIC), for imaging the processive motion of molecular motors myosin V and VI along actin filaments. Both technologies will allow imaging in the presence of higher fluorophore concentrations than TIRF microscopy. They will enable new biophysical measurements of a wide range of processive molecular motors that move along filamentous tracks, such as other myosins, dynein, and kinesin. A particularly salient application of these technologies will be to examine chemomechanical coupling by directly imaging fluorescent nucleotide molecules interacting with processive motors as they traverse their actin or microtubule tracks.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.