ArticleGenes & development2013
Spindle pole body-anchored Kar3 drives the nucleus along microtubules from another nucleus in preparation for nuclear fusion during yeast karyogamy.
Article in Genes & development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 32 citations in OpenAlex.
- The kinesin Kar3 is required for endoplasmic reticulum-associated degradation.Molecular biology of the cell · 2025Article
- Cik1 and Vik1 accessory proteins confer distinct functions to the kinesin-14 Kar3.Journal of cell science · 2023Article
- The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.The Journal of cell biology · 2020Article
- Sporulation inJournal of fungi (Basel, Switzerland) · 2020Review
- Article
- Characterization of spindle pole body duplication reveals a regulatory role for nuclear pore complexes.The Journal of cell biology · 2017Article
- Nuclear migration during karyogamy in rice zygotes is mediated by continuous convergence of actin meshwork toward the egg nucleus.Journal of plant research · 2017Article
- Candida albicans Kinesin Kar3 Depends on a Cik1-Like Regulatory Partner Protein for Its Roles in Mating, Cell Morphogenesis, and Bipolar Spindle Formation.Eukaryotic cell · 2015Article
- Improved Plasmids for Fluorescent Protein Tagging of Microtubules in Saccharomyces cerevisiae.Traffic (Copenhagen, Denmark) · 2015Article
- Cell biology of yeast zygotes, from genesis to budding.Biochimica et biophysica acta · 2015Review
- Kar1 binding to Sfi1 C-terminal regions anchors the SPB bridge to the nuclear envelope.The Journal of cell biology · 2015Article
- The centrosomal linker and microtubules provide dual levels of spatial coordination of centrosomes.PLoS genetics · 2015Article
- Geometrical and mechanical properties control actin filament organization.PLoS computational biology · 2015Article
- Microtubule minus end motors kinesin-14 and dynein drive nuclear congression in parallel pathways.The Journal of cell biology · 2015Article
- Article
- Absence of SUN-domain protein Slp1 blocks karyogamy and switches meiotic recombination and synapsis from homologs to sister chromatids.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Dynamics of Male and Female Chromatin during Karyogamy in Rice Zygotes.Plant physiology · 2014Article
- Molecular determinants of sporulation in Ashbya gossypii.Genetics · 2013Article
- Genomes of Ashbya fungi isolated from insects reveal four mating-type loci, numerous translocations, lack of transposons, and distinct gene duplications.G3 (Bethesda, Md.) · 2013Article
- Proper microtubule structure is vital for timely progression through meiosis in fission yeast.PloS one · 2013Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Nuclear migration during yeast karyogamy, termed nuclear congression, is required to initiate nuclear fusion. Congression involves a specific regulation of the microtubule minus end-directed kinesin-14 motor Kar3 and a rearrangement of the cytoplasmic microtubule attachment sites at the spindle pole bodies (SPBs). However, how these elements interact to produce the forces necessary for nuclear migration is less clear. We used electron tomography, molecular genetics, quantitative imaging, and first principles modeling to investigate how cytoplasmic microtubules are organized during nuclear congression. We found that Kar3, with the help of its light chain, Cik1, is anchored during mating to the SPB component Spc72 that also serves as a nucleator and anchor for microtubules via their minus ends. Moreover, we show that no direct microtubule-microtubule interactions are required for nuclear migration. Instead, SPB-anchored Kar3 exerts the necessary pulling forces laterally on microtubules emanating from the SPB of the mating partner nucleus. Therefore, a twofold symmetrical application of the core principle that drives nuclear migration in higher cells is used in yeast to drive nuclei toward each other before nuclear fusion.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.