ArticleJournal of virology2013
Alphavirus genome delivery occurs directly at the plasma membrane in a time- and temperature-dependent process.
Article in Journal of virology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
25 citing papers in PubMed, 43 citations in OpenAlex.
- Arthropod-borne encephalitis: an overview for the clinician and emerging considerations.Postgraduate medical journal · 2023Review
- Colocalization of Chikungunya Virus with Its Receptor MXRA8 during Cell Attachment, Internalization, and Membrane Fusion.Journal of virology · 2023Article
- Advances in the Development of Small Molecule Antivirals against Equine Encephalitic Viruses.Viruses · 2023Review
- Members of Venezuelan Equine Encephalitis complex entry into host cells by clathrin-mediated endocytosis in a pH-dependent manner.Scientific reports · 2022Article
- Enhancing Antibodies' Binding Capacity through Oriented Functionalization of Plasmonic Surfaces.Nanomaterials (Basel, Switzerland) · 2021Article
- Microbes increase thermal sensitivity in the mosquito Aedes aegypti, with the potential to change disease distributions.PLoS neglected tropical diseases · 2021Article
- UsingJournal of virology · 2021Review
- Review
- A molecular understanding of alphavirus entry.PLoS pathogens · 2020Review
- TheViruses · 2020Article
- The Role of Temperature in Shaping Mosquito-Borne Viruses Transmission.Frontiers in microbiology · 2020Review
- Purification and Proteomic Analysis of Alphavirus Particles from Sindbis Virus Grown in Mammalian and Insect Cells.Bio-protocol · 2019Article
- Viral journeys on the intracellular highways.Cellular and molecular life sciences : CMLS · 2018Review
- Guiding protein delivery into live cells using DNA-programmed membrane fusion.Chemical science · 2018Article
- Comparative Characterization of the Sindbis Virus Proteome from Mammalian and Invertebrate Hosts Identifies nsP2 as a Component of the Virion and Sorting Nexin 5 as a Significant Host Factor for Alphavirus Replication.Journal of virology · 2018Article
- Role of the vacuolar ATPase in theHeliyon · 2018Article
- Review
- Herpes Simplex Virus 1 Enters Human Keratinocytes by a Nectin-1-Dependent, Rapid Plasma Membrane Fusion Pathway That Functions at Low Temperature.Journal of virology · 2016Article
- Single-Site Glycoprotein Mutants Inhibit a Late Event in Sindbis Virus Assembly.Journal of virology · 2016Article
- Early Events in Chikungunya Virus Infection-From Virus Cell Binding to Membrane Fusion.Viruses · 2015Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
It is widely held that arboviruses such as the alphavirus Sindbis virus gain entry into cells by a process of receptor-mediated endocytosis followed by membrane fusion in the acid environment of the endosome. We have used an approach of direct observation of Sindbis virus entry into cells by electron microscopy and immunolabeling of virus proteins with antibodies conjugated to gold beads. We found that upon attaching to the cell surface, intact RNA-containing viruses became empty shells that could be identified only by antibody labeling. We found that the rate at which full particles were converted to empty particles increased with time and temperature. We found that this entry event takes place at temperatures that inhibit both endosome formation and membrane fusion. We conclude that entry of alphaviruses is by direct penetration of cell plasma membranes through a pore structure formed by virus and, possibly, host proteins.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.