ArticleIntegrative biology : quantitative biosciences from nano to macro2013
Microfabricated collagen tracks facilitate single cell metastatic invasion in 3D.
Article in Integrative biology : quantitative biosciences from nano to macro, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
What it found
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Who cites it
70 citing papers in PubMed, 115 citations in OpenAlex.
- Multicellular force coordination constructs microchannel networks for barrier-free metastasis across extracellular matrix.Science advances · 2025Article
- Engineered In Vitro Platforms for Mechanochemical Control of Cell Migration.Cold Spring Harbor perspectives in biology · 2025Review
- Confinement by Liquid-Liquid Interface Replicates In Vivo Neutrophil Deformations and Elicits Bleb-Based Migration.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Article
- Regulation of cell migration by extracellular matrix mechanics at a glance.Journal of cell science · 2025Review
- Hydrogel Design to Understand and Guide 3D Cell Migration.Regenerative engineering and translational medicine · 2025Review
- Confinement-sensitive volume regulation dynamics via high-speed nuclear morphological measurements.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Recent advances in shape memory scaffolds and regenerative outcomes.Biomedical engineering letters · 2024Review
- Microinterfaces in biopolymer-based bicontinuous hydrogels guide rapid 3D cell migration.Nature communications · 2024Article
- Mechanically primed cells transfer memory to fibrous matrices for invasion across environments of distinct stiffness and dimensionality.Molecular biology of the cell · 2023Article
- Link between glucose metabolism and epithelial-to-mesenchymal transition drives triple-negative breast cancer migratory heterogeneity.iScience · 2022Article
- Cancer-Associated Fibroblasts in a 3D Engineered Tissue Model Induce Tumor-like Matrix Stiffening and EMT Transition.Cancers · 2022Article
- Engineered colorectal cancer tissue recapitulates key attributes of a patient-derived xenograft tumor line.Biofabrication · 2022Article
- Cancer cell mechanobiology: a new frontier for cancer research.Journal of the National Cancer Center · 2022Review
- T Cells Promote Metastasis by Regulating Extracellular Matrix Remodeling following Chemotherapy.Cancer research · 2022Article
- Matricellular Protein WISP2 Is an Endogenous Inhibitor of Collagen Linearization and Cancer Metastasis.Cancer research · 2021Article
- Phenotypic Heterogeneity and Metastasis of Breast Cancer Cells.Cancer research · 2021Article
- Learning the dynamics of cell-cell interactions in confined cell migration.Proceedings of the National Academy of Sciences of the United States of America · 2021Article
- Transduction of cell and matrix geometric cues by the actin cytoskeleton.Current opinion in cell biology · 2021Review
- Molecular and functional extracellular vesicle analysis using nanopatterned microchips monitors tumor progression and metastasis.Science translational medicine · 2020Article
- Biosensors for Studies on Adhesion-Mediated Cellular Responses to Their Microenvironment.Frontiers in bioengineering and biotechnology · 2020Review
10 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
While the mechanisms employed by metastatic cancer cells to migrate remain poorly understood, it has been widely accepted that metastatic cancer cells can invade the tumor stroma by degrading the extracellular matrix (ECM) with matrix metalloproteinases (MMPs). Although MMP inhibitors showed early promise in preventing metastasis in animal models, they have largely failed clinically. Recently, studies have shown that some cancer cells can use proteolysis to mechanically rearrange their ECM to form tube-like "microtracks" which other cells can follow without using MMPs themselves. We speculate that this mode of migration in the secondary cells may be one example of migration which can occur without endogenous protease activity in the secondary cells. Here we present a technique to study this migration in a 3D, collagen-based environment which mimics the size and topography of the tracks produced by proteolytically active cancer cells. Using time-lapse phase-contrast microscopy, we find that these microtracks permit the rapid and persistent migration of noninvasive MCF10A mammary epithelial cells, which are unable to otherwise migrate in 3D collagen. Additionally, while highly metastatic MDAMB231 breast cancer cells are able to invade a 3D collagen matrix, seeding within the patterned microtracks induced significantly increased cell migration speed, which was not decreased by pharmacological MMP inhibition. Together, these data suggest that microtracks within a 3D ECM may facilitate the migration of cells in an MMP-independent fashion, and may reveal novel insight into the clinical challenges facing MMP inhibitors.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.