ArticleCell cycle (Georgetown, Tex.)2013
Nature of KaiB-KaiC binding in the cyanobacterial circadian oscillator.
Article in Cell cycle (Georgetown, Tex.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
12 citing papers in PubMed, 18 citations in OpenAlex.
- Architecture and mechanism of the central gear in an ancient molecular timer.Journal of the Royal Society, Interface · 2017Review
- A dynamic interaction process between KaiA and KaiC is critical to the cyanobacterial circadian oscillator.Scientific reports · 2016Article
- Daily expression pattern of protein-encoding genes and small noncoding RNAs in synechocystis sp. strain PCC 6803.Applied and environmental microbiology · 2014Article
- Intricate protein-protein interactions in the cyanobacterial circadian clock.The Journal of biological chemistry · 2014Review
- An arginine tetrad as mediator of input-dependent and input-independent ATPases in the clock protein KaiC.Acta crystallographica. Section D, Biological crystallography · 2014Article
- Insight into cyanobacterial circadian timing from structural details of the KaiB-KaiC interaction.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Cooperative KaiA-KaiB-KaiC interactions affect KaiB/SasA competition in the circadian clock of cyanobacteria.Journal of molecular biology · 2014Article
- Metabolic compensation and circadian resilience in prokaryotic cyanobacteria.Annual review of biochemistry · 2014Review
- A circadian clock nanomachine that runs without transcription or translation.Current opinion in neurobiology · 2013Review
- CryoEM and molecular dynamics of the circadian KaiB-KaiC complex indicates that KaiB monomers interact with KaiC and block ATP binding clefts.Journal of molecular biology · 2013Article
- Nuclear magnetic resonance spectroscopy of the circadian clock of cyanobacteria.Integrative and comparative biology · 2013Review
- KaiC intersubunit communication facilitates robustness of circadian rhythms in cyanobacteria.Nature communications · 2013Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 1 country.
Funding
Abstract
In the cyanobacteria Synechococcus elongatus and Thermosynechococcus elongatus, the KaiA, KaiB and KaiC proteins in the presence of ATP generate a post-translational oscillator (PTO) that can be reconstituted in vitro. KaiC is the result of a gene duplication and resembles a double doughnut with N-terminal CI and C-terminal CII hexameric rings. Six ATPs are bound between subunits in both the CI and CII ring. CI harbors ATPase activity, and CII catalyzes phosphorylation and dephosphorylation at T432 and S431 with a ca. 24-h period. KaiA stimulates KaiC phosphorylation, and KaiB promotes KaiC subunit exchange and sequesters KaiA on the KaiB-KaiC interface in the final stage of the clock cycle. Studies of the PTO protein-protein interactions are convergent in terms of KaiA binding to CII but have led to two opposing models of the KaiB-KaiC interaction. Electron microscopy (EM) and small angle X-ray scattering (SAXS), together with native PAGE using full-length proteins and separate CI and CII rings, are consistent with binding of KaiB to CII. Conversely, NMR together with gel filtration chromatography and denatured PAGE using monomeric CI and CII domains support KaiB binding to CI. To resolve the existing controversy, we studied complexes between KaiB and gold-labeled, full-length KaiC with negative stain EM. The EM data clearly demonstrate that KaiB contacts the CII ring. Together with the outcomes of previous analyses, our work establishes that only CII participates in interactions with KaiA and KaiB as well as with the His kinase SasA involved in the clock output pathway.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.