ArticleStem cells and development2013
Growth differentiation factor-5 enhances in vitro mesenchymal stromal cell chondrogenesis and hypertrophy.
Article in Stem cells and development, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Intra-articular injection of stromal vascular fraction for knee degenerative joint disease: a concise review of preclinical and clinical evidence.Science China. Life sciences · 2022Pooled it
- Donor-dependent regulation of type II and X collagen deposition by early modulation of miR-335-5p and miR-1246 during chondrogenic commitment.Stem cell research & therapy · 2025Article
- Polymorphism rs143384 GDF5 reduces the risk of knee osteoarthritis development in obese individuals and increases the disease risk in non-obese population.Arthroplasty (London, England) · 2024Article
- Collagen in the central nervous system: contributions to neurodegeneration and promise as a therapeutic target.Molecular neurodegeneration · 2024Review
- Article
- Bioactivity of human adult stem cells and functional relevance of stem cell-derived extracellular matrix in chondrogenesis.Stem cell research & therapy · 2023Article
- Cartilage 3D bioprinting for rhinoplasty using adipose-derived stem cells as seed cells: Review and recent advances.Cell proliferation · 2023Review
- Comparative in vitro treatment of mesenchymal stromal cells with GDF-5 and R57A induces chondrogenic differentiation while limiting chondrogenic hypertrophy.Journal of experimental orthopaedics · 2023Article
- Regulatory Mechanisms ofInternational journal of molecular sciences · 2022Review
- Current Insights Into the Maintenance of Structure and Function of Intervertebral Disc: A Review of the Regulatory Role of Growth and Differentiation Factor-5.Frontiers in pharmacology · 2022Review
- Preparation and Application of Decellularized ECM-Based Biological Scaffolds for Articular Cartilage Repair: A Review.Frontiers in bioengineering and biotechnology · 2022Review
- Growth differentiation factor 5 in cartilage and osteoarthritis: A possible therapeutic candidate.Cell proliferation · 2021Review
- GDF-5 promotes epidermal stem cells proliferation via Foxg1-cyclin D1 signaling.Stem cell research & therapy · 2021Article
- The GDF-5 mutant M1673 exerts robust anabolic and anti-catabolic effects in chondrocytes.Journal of cellular and molecular medicine · 2020Article
- Adipose tissue-derived mesenchymal stem cells and chitosan/poly (vinyl alcohol) nanofibrous scaffolds for cartilage tissue engineering.Cell regeneration (London, England) · 2020Article
- GDF5 induces TBX3 in a concentration dependent manner - on a gold nanoparticle gradient.Heliyon · 2020Article
- Mechanisms of synovial joint and articular cartilage development.Cellular and molecular life sciences : CMLS · 2019Review
- Hypoxia Activates the PTHrP -MEF2C Pathway to Attenuate Hypertrophy in Mesenchymal Stem Cell Derived Cartilage.Scientific reports · 2019Article
- Comparison of the effect of growth factors on chondrogenesis of canine mesenchymal stem cells.The Journal of veterinary medical science · 2019Article
- Therapeutic potential of growth differentiation factors in the treatment of degenerative disc diseases.JOR spine · 2019Review
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regenerative potential for adult bone marrow-derived mesenchymal stromal cells (MSCs) has been extensively investigated in the setting of arthritic disease and focal cartilage defects. In vitro chondrogenic differentiation of MSCs is regularly accomplished by the widely used pellet culture system where MSCs are maintained in high-density pellets to mimic mesenchymal condensation during development. Supplementation of chondrogenic MSC pellet cultures with growth differentiation factor-5 (GDF-5), a highly regulated gene in the chondrogenic phase of endochondral ossification (EO), was investigated here under the hypothesis that GDF-5 will enhance the chondrogenic differentiation of MSCs, thereby supporting their entry into ossification. The supplementation of chondrogenic MSC pellets with the recombinant human GDF-5 protein significantly enhanced MSC chondrogenic differentiation, as demonstrated by enhanced collagen type II and sulfated glycosaminoglycan (GAG) incorporation into the extracellular matrix. Increased P-SMADs 1-5-8 were observed in pellets treated with GDF-5 and transforming growth factor (TGF)-β 3 when compared to the pellets treated with TGF-β 3 alone, demonstrated by immunostaining and western blot analysis of the chondrogenic pellet extract. A concurrent increase in alkaline phosphatase, collagen types I and X, and osteopontin secretion indicated a transition of these cultures to hypertrophy. Together, these data support the application of GDF-5 to enhance MSC chondrogenic differentiation and hypertrophy as a precursor to EO.
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