ArticleAnalytical chemistry2013
An automated high-throughput counting method for screening circulating tumor cells in peripheral blood.
Article in Analytical chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed.
- Autonomous microfluidic labs: progress and prospects.Lab on a chip · 2026Review
- Comparative Analysis of Sample Loop and Counting Bead-Based Methods for Size-Dependent Bias in Flow Cytometry.Analytical chemistry · 2025Article
- Novel Isolating Approaches to Circulating Tumor Cell Enrichment Based on Microfluidics: A Review.Micromachines · 2024Review
- Clinical significance of genomic sequencing of circulating tumour cells (CTCs) in cancer.The journal of liquid biopsy · 2024Review
- Enrichment and ratiometric detection of circulating tumor cells using PSMA- and folate receptor-targeted magnetic and surface-enhanced Raman scattering nanoparticles.Biomedical optics express · 2020Article
- Label-free detection of rare circulating tumor cells by image analysis and machine learning.Scientific reports · 2020Article
- Computational cytometer based on magnetically modulated coherent imaging and deep learning.Light, science & applications · 2019Article
- Integrated Microfluidic Device for Enrichment and Identification of Circulating Tumor Cells from the Blood of Patients with Colorectal Cancer.Disease markers · 2019Article
- Microfluidic Chip with Integrated Electrophoretic Immunoassay for Investigating Cell-Cell Interactions.Analytical chemistry · 2018Article
- NanoVelcro rare-cell assays for detection and characterization of circulating tumor cells.Advanced drug delivery reviews · 2018Review
- Current detection technologies for circulating tumor cells.Chemical Society reviews · 2017Review
- Translating microfluidics: Cell separation technologies and their barriers to commercialization.Cytometry. Part B, Clinical cytometry · 2017Review
- Immunomagnetic separation of tumor initiating cells by screening two surface markers.Scientific reports · 2017Article
- Circulating tumor cells: A promising marker of predicting tumor response in rectal cancer patients receiving neoadjuvant chemo-radiation therapy.Oncotarget · 2016Article
- Clinical Applications of NanoVelcro Rare-Cell Assays for Detection and Characterization of Circulating Tumor Cells.Theranostics · 2016Review
- Photoresponsive immunomagnetic nanocarrier for capture and release of rare circulating tumor cells.Chemical science · 2015Article
- An electrochemical immunosensing method for detecting melanoma cells.Biosensors & bioelectronics · 2015Article
- A novel method for downstream characterization of breast cancer circulating tumor cells following CellSearch isolation.Journal of translational medicine · 2015Article
- Microfluidic cell sorting: a review of the advances in the separation of cells from debulking to rare cell isolation.Lab on a chip · 2015Review
- Advancing the speed, sensitivity and accuracy of biomolecular detection using multi-length-scale engineering.Nature nanotechnology · 2014Review
Corrections and comments
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Authors and funding
8 authors.
Funding
Abstract
Enumeration of circulating tumor cells (CTCs) has proved valuable for early detection and prognosis in cancer treatment. This paper describes an automated high-throughput counting method for CTCs based on microfluidics and line-confocal microscopy. Peripheral blood was directly labeled with multiple antibodies, each conjugated with a different fluorophore, pneumatically pumped through a microfluidic channel, and interrogated by a line-confocal microscope. On the basis of the fluorescence signals and labeling schemes, the count of CTCs was automatically reported. Due to the high flow rate, 1 mL of whole blood can be analyzed in less than 30 min. We applied this method in analyzing CTCs from 90 stage IV breast cancer patient samples and performed a side-by-side comparison with the results of the CellSearch assay, which is the only method approved by the U.S. Food and Drug Administration at present for enumeration of CTCs. This method has a recovery rate for cultured breast cancer cells of 94% (n = 9), with an average of 1.2 counts/mL of background level of detected CTCs from healthy donors. It detected CTCs from breast cancer patients ranging from 15 to 3375 counts/7.5 mL. Using this method, we also demonstrate the ability to enumerate CTCs from breast cancer patients that were positive for Her2 or CD44(+)/CD24(-), which is a putative cancer stem cell marker. This automated method can enumerate CTCs from peripheral blood with high throughput and sensitivity. It could potentially benefit the clinical diagnosis and prognosis of cancer.
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Registered trials
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