ArticleThe Journal of biological chemistry2013
A nibbling mechanism for clathrin-mediated retrieval of secretory granule membrane after exocytosis.
Article in The Journal of biological chemistry, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 27 citations in OpenAlex.
- Forty years of the adrenal chromaffin cell through ISCCB meetings around the world.Pflugers Archiv : European journal of physiology · 2023Review
- Cell-specific secretory granule sorting mechanisms: the role of MAGEL2 and retromer in hypothalamic regulated secretion.Frontiers in cell and developmental biology · 2023Review
- Illuminating membrane structural dynamics of fusion and endocytosis with advanced light imaging techniques.Biochemical Society transactions · 2022Review
- A mechanism of self-lipid endocytosis mediated by the receptor Mincle.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Vesicle Shrinking and Enlargement Play Opposing Roles in the Release of Exocytotic Contents.Cell reports · 2020Article
- Chromogranin A, the major lumenal protein in chromaffin granules, controls fusion pore expansion.The Journal of general physiology · 2019Article
- Visualization of expanding fusion pores in secretory cells.The Journal of general physiology · 2018Review
- Regulation of insulin exocytosis by calcium-dependent protein kinase C in beta cells.Cell calcium · 2017Review
- Slow fusion pore expansion creates a unique reaction chamber for co-packaged cargo.The Journal of general physiology · 2017Article
- Imaging the recruitment and loss of proteins and lipids at single sites of calcium-triggered exocytosis.Molecular biology of the cell · 2016Article
- Dynamin 2 regulates biphasic insulin secretion and plasma glucose homeostasis.The Journal of clinical investigation · 2015Article
- Distinct fusion properties of synaptotagmin-1 and synaptotagmin-7 bearing dense core granules.Molecular biology of the cell · 2014Article
- Lumenal protein within secretory granules affects fusion pore expansion.Biophysical journal · 2014Article
- Systematic spatial mapping of proteins at exocytic and endocytic structures.Molecular biology of the cell · 2014Article
- Synthesis of Dynole 34-2, Dynole 2-24 and Dyngo 4a for investigating dynamin GTPase.Nature protocols · 2014Article
- Lymphoid aggregates in Crohn's colitis and mucosal immunity.Virchows Archiv : an international journal of pathology · 2013Article
- Exocytosis and endocytosis in neuroendocrine cells: inseparable membranes!Frontiers in endocrinology · 2013Review
- Carcinoma in gut-associated lymphoid tissue in ulcerative colitis: Case report and review of literature.World journal of gastrointestinal endoscopy · 2013Article
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Clathrin-mediated endocytosis is the major pathway for recycling of granule membrane components after strong stimulation and high exocytotic rates. It resembles "classical" receptor-mediated endocytosis but has a trigger that is unique to secretion, the sudden appearance of the secretory granule membrane in the plasma membrane. The spatial localization, the relationship to individual fusion events, the nature of the cargo, and the timing and nature of the nucleation events are unknown. Furthermore, a size mismatch between chromaffin granules (∼300-nm diameter) and typical clathrin-coated vesicles (∼90 nm) makes it unlikely that clathrin-mediated endocytosis internalizes as a unit the entire fused granule membrane. We have used a combination of total internal reflection fluorescence microscopy of transiently expressed proteins and time-resolved quantitative confocal imaging of endogenous proteins along with a fluid-phase marker to address these issues. We demonstrate that the fused granule membrane remains a distinct entity and serves as a nucleation site for clathrin- and dynamin-mediated endocytosis that internalizes granule membrane components in small increments.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.