ArticleMethods in molecular biology (Clifton, N.J.)2013
Functional genomic analysis of cotton genes with agrobacterium-mediated virus-induced gene silencing.
Article in Methods in molecular biology (Clifton, N.J.), 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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25 citing papers in PubMed, 51 citations in OpenAlex.
- Transcriptional profiling of anthocyanin biosynthesis in variegated and non-variegated dorsal petal regions of Impatiens uliginosa.BMC plant biology · 2026Article
- The identification of gibberellin 2-oxidase gene family in cotton and functional analysis.BMC plant biology · 2026Article
- Comparative single-cell transcriptomic map reveals divergence in leaves between two cotton species at cell type resolution.Journal of advanced research · 2026Article
- Genome-wide characterization of the cotton E2F/DP gene family and a negative role of GhDEL1_D08 in salt tolerance.BMC plant biology · 2025Article
- Genome-Wide Characterization of theInternational journal of molecular sciences · 2025Article
- Genome-wide identification of VOZ gene family in ten cotton species and the function analysis of GhVOZ2 involved in heat stress response.BMC genomics · 2025Article
- The potato StMKK5-StSIPK module enhances resistance to Phytophthora pathogens through activating the salicylic acid and ethylene signalling pathways.Molecular plant pathology · 2023Article
- Fatty acid amide hydrolase and 9-lipoxygenase modulate cotton seedling growth by ethanolamide oxylipin levels.Plant physiology · 2023Article
- Article
- California TRV-based VIGS vectors mediate gene silencing at elevated temperatures but with greater growth stunting.BMC plant biology · 2021Article
- A stable QTL qSalt-A04-1 contributes to salt tolerance in the cotton seed germination stage.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2021Article
- Genome-Wide Study of NOT2_3_5 Protein Subfamily in Cotton and Their Necessity in Resistance toInternational journal of molecular sciences · 2021Article
- Gene Expression Correlation Analysis Reveals MYC-NAC Regulatory Network in Cotton Pigment Gland Development.International journal of molecular sciences · 2021Article
- Molecular cloning and characterization of GhERF105, a gene contributing to the regulation of gland formation in upland cotton (Gossypium hirsutum L.).BMC plant biology · 2021Article
- Genome-Wide Identification of theFrontiers in genetics · 2021Article
- Review
- Molecular insight into cotton leaf curl geminivirus disease resistance in cultivated cotton (Gossypium hirsutum).Plant biotechnology journal · 2020Article
- Genome wide identification of the trihelix transcription factors and overexpression of Gh_A05G2067 (GT-2), a novel gene contributing to increased drought and salt stresses tolerance in cotton.Physiologia plantarum · 2019Article
- Role of a cotton endoreduplication-related gene, GaTOP6B, in response to drought stress.Planta · 2019Article
- Genome-wide identification and characterization of LRR-RLKs reveal functional conservation of the SIF subfamily in cotton (Gossypium hirsutum).BMC plant biology · 2018Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Cotton (Gossypium spp.) is one of the most agronomically important crops worldwide for its unique textile fiber production and serving as food and feed stock. Molecular breeding and genetic engineering of useful genes into cotton have emerged as advanced approaches to improve cotton yield, fiber quality, and resistance to various stresses. However, the understanding of gene functions and regulations in cotton is largely hindered by the limited molecular and biochemical tools. Here, we describe the method of an Agrobacterium infiltration-based virus-induced gene silencing (VIGS) assay to transiently silence endogenous genes in cotton at 2-week-old seedling stage. The genes of interest could be readily silenced with a consistently high efficiency. To monitor gene silencing efficiency, we have cloned cotton GrCla1 from G. raimondii, a homolog gene of Arabidopsis Cloroplastos alterados 1 (AtCla1) involved in chloroplast development, and inserted into a tobacco rattle virus (TRV) binary vector pYL156. Silencing of GrCla1 results in albino phenotype on the newly emerging leaves, serving as a visual marker for silencing efficiency. To further explore the possibility of using VIGS assay to reveal the essential genes mediating disease resistance to Verticillium dahliae, a fungal pathogen causing severe Verticillium wilt in cotton, we developed a seedling infection assay to inoculate cotton seedlings when the genes of interest are silenced by VIGS. The method we describe here could be further explored for functional genomic analysis of cotton genes involved in development and various biotic and abiotic stresses.
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