ArticleActa crystallographica. Section F, Structural biology and crystallization communications2013
Some practical guidelines for UV imaging in the protein crystallization laboratory.
Article in Acta crystallographica. Section F, Structural biology and crystallization communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Comprehensive microcrystal electron diffraction sample preparation for cryo-EM.Nature protocols · 2025Review
- Direct Imaging of the Kinetic Crystallization Pathway: Simulation and Liquid-Phase Transmission Electron Microscopy Observations.Materials (Basel, Switzerland) · 2023Review
- Moving beyond MARCO.PloS one · 2023Article
- Inexpensive robotic system for standard and fluorescent imaging of protein crystals.Acta crystallographica. Section F, Structural biology communications · 2019Article
- Strategies for sample delivery for femtosecond crystallography.Acta crystallographica. Section D, Structural biology · 2019Review
- Improved protein-crystal identification by using 2,2,2-trichloroethanol as a fluorescence enhancer.Acta crystallographica. Section F, Structural biology communications · 2018Article
- Transmission electron microscopy for the evaluation and optimization of crystal growth.Acta crystallographica. Section D, Structural biology · 2016Article
- A visible-light-excited fluorescence method for imaging protein crystals without added dyes.Journal of applied crystallography · 2016Article
- Identifying, studying and making good use of macromolecular crystals.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Use of transmission electron microscopy to identify nanocrystals of challenging protein targets.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Approaches to automated protein crystal harvesting.Acta crystallographica. Section F, Structural biology communications · 2014Article
- Introduction to protein crystallization.Acta crystallographica. Section F, Structural biology communications · 2014Review
- An overview of biological macromolecule crystallization.International journal of molecular sciences · 2013Review
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3 authors.
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Abstract
High-throughput imaging of protein crystallization experiments with ultraviolet (UV) light has recently become commercially available and can enable crystallographers to differentiate between crystals of protein and those of salt, as the visualization of protein crystals is based on intrinsic tryptophan fluorescence. Unfortunately, UV imaging is not a panacea, as some protein crystals will not fluoresce under UV excitation and some salt crystals are UV-fluorescently active. As a new technology, there is little experience within the general community on how to use this technology effectively and what caveats to look out for. Here, an attempt is made to identify some of the common problems that may arise using UV-imaging technology by examining test proteins, common crystallization reagents and a range of proteins by assessing their UV-Vis absorbance spectra. Some pointers are offered as to which systems may not be appropriate for this methodology.
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