ArticleNature communications2013
Transferring a synthetic gene circuit from yeast to mammalian cells.
Article in Nature communications, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 56 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
56 citing papers in PubMed.
- Programmable promoter editing for precise control of transgene expression.Nature biotechnology · 2026Article
- Mammalian synthetic gene circuits for biopharmaceutical development & manufacture.NPJ systems biology and applications · 2025Review
- A single vector system for tunable and homogeneous dual gene expression in Escherichia coli.Scientific reports · 2025Article
- Perspectives on Synthetic Protein Circuits in Mammalian Cells.Current opinion in biomedical engineering · 2024Article
- Optimizing a CRISPR-Cas13d Gene Circuit for Tunable Target RNA Downregulation with Minimal Collateral RNA Cutting.ACS synthetic biology · 2024Article
- Synthetic gene circuit evolution: Insights and opportunities at the mid-scale.Cell chemical biology · 2024Review
- Programmable promoter editing for precise control of transgene expression.bioRxiv : the preprint server for biology · 2024Article
- EMT transcription factors activated circuits: A novel tool to study EMT dynamics and its therapeutic implications.Synthetic and systems biotechnology · 2024Article
- Adaptive DNA amplification of synthetic gene circuit opens a way to overcome cancer chemoresistance.Proceedings of the National Academy of Sciences of the United States of America · 2023Article
- Nonmonotone invasion landscape by noise-aware control of metastasis activator levels.Nature chemical biology · 2023Article
- CasTuner is a degron and CRISPR/Cas-based toolkit for analog tuning of endogenous gene expression.Nature communications · 2023Article
- Synthetic memory circuits for stable cell reprogramming in plants.Nature biotechnology · 2022Article
- Engineering of Synthetic Transcriptional Switches in Yeast.Life (Basel, Switzerland) · 2022Review
- Design and characterization of a salicylic acid-inducible gene expression system for Jurkat cells.Journal of biotechnology · 2022Article
- A Platform Technology for Monitoring the Unfolded Protein Response.Methods in molecular biology (Clifton, N.J.) · 2022Article
- Gene-circuit therapy on the horizon: synthetic biology tools for engineered therapeutics.Acta biochimica Polonica · 2021Review
- Quantitative control of noise in mammalian gene expression by dynamic histone regulation.eLife · 2021Article
- Reliably Engineering and Controlling Stable Optogenetic Gene Circuits in Mammalian Cells.Journal of visualized experiments : JoVE · 2021Article
- A synthetic circuit for buffering gene dosage variation between individual mammalian cells.Nature communications · 2021Article
- Context-aware synthetic biology by controller design: Engineering the mammalian cell.Cell systems · 2021Review
Corrections and comments
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Authors and funding
3 authors.
Funding
Abstract
The emerging field of synthetic biology builds gene circuits for scientific, industrial and therapeutic needs. Adaptability of synthetic gene circuits across different organisms could enable a synthetic biology pipeline, where circuits are designed in silico, characterized in microbes and reimplemented in mammalian settings for practical usage. However, the processes affecting gene circuit adaptability have not been systematically investigated. Here we construct a mammalian version of a negative feedback-based 'linearizer' gene circuit previously developed in yeast. The first naïve mammalian prototype was non-functional, but a computational model suggested that we could recover function by improving gene expression and protein localization. After rationally developing and combining new parts as the model suggested, we regained function and could tune target gene expression in human cells linearly and precisely as in yeast. The steps we have taken should be generally relevant for transferring any gene circuit from yeast into mammalian cells.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.