ArticleEnvironmental health perspectives2013
Performance in omics analyses of blood samples in long-term storage: opportunities for the exploitation of existing biobanks in environmental health research.
Article in Environmental health perspectives, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers, 1 of them a synthesis that pooled it.
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Who cites it
74 citing papers in PubMed, 1 synthesis or guideline pooled it, 149 citations in OpenAlex.
- Guidelines for pre-analytical conditions for assessing the methylation of circulating cell-free DNA.Clinical epigenetics · 2021Pooled it
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- Metabolomic Alterations in Patients with Obesity and the Impact of Metabolic Bariatric Surgery: Insights for Future Research.Metabolites · 2025Review
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- Assessing the influence of sleep and sampling time on metabolites in oral fluid: implications for metabolomics studies.Metabolomics : Official journal of the Metabolomic Society · 2024Article
- Characteristics of RNA Stabilizer RNApro for Peripheral Blood Collection.Diagnostics (Basel, Switzerland) · 2024Article
- Uveal Melanoma Patients Have a Distinct Metabolic Phenotype in Peripheral Blood.International journal of molecular sciences · 2023Article
- Multi-omic comparative analysis of COVID-19 and bacterial sepsis-induced ARDS.PLoS pathogens · 2022Article
- Multi-omic comparative analysis of COVID-19 and bacterial sepsis-induced ARDS.medRxiv : the preprint server for health sciences · 2022Article
- Peripheral blood DNA methylation profiles predict future development of B-cell Non-Hodgkin Lymphoma.NPJ precision oncology · 2022Article
- Metabolomic signatures of low- and high-adiposity neonates differ based on maternal BMI.American journal of physiology. Endocrinology and metabolism · 2022Article
- The impact of pre-freezing storage time and temperature on gene expression of blood collected in EDTA tubes.Molecular biology reports · 2022Article
- Prenatal Exposure to Heavy Metals Affects Gestational Age by Altering DNA Methylation Patterns.Nanomaterials (Basel, Switzerland) · 2021Article
- Novel Serum and Urinary Metabolites Associated with Diabetic Retinopathy in Three Asian Cohorts.Metabolites · 2021Article
14 more citing papers are in PubMed but not listed here.
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Authors and funding
20 authors at 11 institutions in 6 countries.
Funding
Abstract
backgroundThe suitability for omic analysis of biosamples collected in previous decades and currently stored in biobanks is unknown.
objectivesWe evaluated the influence of handling and storage conditions of blood-derived biosamples on transcriptomic, epigenomic (CpG methylation), plasma metabolomic [UPLC-ToFMS (ultra performance liquid chromatography-time-of-flight mass spectrometry)], and wide-target proteomic profiles.
methodsWe collected fresh blood samples without RNA preservative in heparin, EDTA, or citrate and held them at room temperature for ≤ 24 hr before fractionating them into buffy coat, erythrocytes, and plasma and freezing the fractions at -80oC or in liquid nitrogen. We developed methodology for isolating RNA from the buffy coats and conducted omic analyses. Finally, we analyzed analogous samples from the EPIC-Italy and Northern Sweden Health and Disease Study biobanks.
resultsMicroarray-quality RNA could be isolated from buffy coats (including most biobank samples) that had been frozen within 8 hr of blood collection by thawing the samples in RNA preservative. Different anticoagulants influenced the metabolomic, proteomic, and to a lesser extent transcriptomic profiles. Transcriptomic profiles were most affected by the delay (as little as 2 hr) before blood fractionation, whereas storage temperature had minimal impact. Effects on metabolomic and proteomic profiles were noted in samples processed ≥ 8 hr after collection, but no effects were due to storage temperature. None of the variables examined significantly influenced the epigenomic profiles. No systematic influence of time-in-storage was observed in samples stored over a period of 13-17 years.
conclusionsMost samples currently stored in biobanks are amenable to meaningful omics analysis, provided that they satisfy collection and storage criteria defined in this study.
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