ArticleDevelopmental biology2013
Polarized Cdc42 activation promotes polar body protrusion and asymmetric division in mouse oocytes.
Article in Developmental biology, 2013. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 109 citations in OpenAlex.
- The mechanisms of DEPDC7-mediated actin cap establishment and meiosis progression in oocytes.EMBO reports · 2026Article
- Post-Translational Modifications of Tubulin in Oocyte Maturation and Female Infertility.Cytoskeleton (Hoboken, N.J.) · 2026Review
- DEPDC7 couples homologous chromosome segregation to asymmetric cytokinesis through recruiting ARP2/3 complex in oocyte meiosis.Cell communication and signaling : CCS · 2026Article
- From Cytoskeletal Remodeling to Oocyte Quality: The Emerging Role of Mechanics.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026Review
- Ultrastructural Aspects of Oocyte Maturation in Dogs, with Comparative Insights from Cats: Current Evidence and Research Perspectives.Animals : an open access journal from MDPI · 2026Review
- Rab32-based vesicles coordinate mitochondria and actin for spindle migration and organelle rearrangement in oocyte meiosis.Journal of advanced research · 2026Article
- Oocyte Kinetochores at the Nexus of Meiotic Drive and Segregation Fidelity.Advances in experimental medicine and biology · 2026Review
- Mevalonate metabolites boost aged oocyte quality through prenylation of small GTPases.Nature aging · 2025Article
- CaCurrent biology : CB · 2025Article
- Diverse developmental velocities of blastocyst development emerge as early as a few hours after insemination: morphokinetic analysis of fertilisation in days 4-7 blastocysts.Journal of assisted reproduction and genetics · 2025Article
- The Morphogenesis of Sperm Storage Micropockets in the Western Mosquitofish (Animals : an open access journal from MDPI · 2025Article
- MRCK activates mouse oocyte myosin II for spindle rotation and male pronucleus centration.The Journal of cell biology · 2023Article
- Cell fragmentation in mouse preimplantation embryos induced by ectopic activation of the polar body extrusion pathway.The EMBO journal · 2023Article
- Tubulin post-translational modifications in meiosis.Seminars in cell & developmental biology · 2023Review
- Polo-like kinase 1 promotes Cdc42-induced actin polymerization for asymmetric division in oocytes.Open biology · 2023Article
- Initial spindle positioning at the oocyte center protects against incorrect kinetochore-microtubule attachment and aneuploidy in mice.Science advances · 2023Article
- Dual control of formin-nucleated actin assembly by the chromatin and ER in mouse oocytes.Current biology : CB · 2022Article
- Advances in the study of CDC42 in the female reproductive system.Journal of cellular and molecular medicine · 2022Review
- Diversity is the spice of life: An overview of how cytokinesis regulation varies with cell type.Frontiers in cell and developmental biology · 2022Review
- RanGTP and the actin cytoskeleton keep paternal and maternal chromosomes apart during fertilization.The Journal of cell biology · 2021Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
Abstract
Asymmetric meiotic divisions in mammalian oocytes rely on the eccentric positioning of the spindle and the remodeling of the overlying cortex, resulting in the formation of small polar bodies. The mechanism of this cortical polarization, exemplified by the formation of a thick F-actin cap, is poorly understood. Cdc42 is a major player in cell polarization in many systems; however, the spatio-temporal dynamics of Cdc42 activation during oocyte meiosis, and its contribution to mammalian oocyte polarization, have remained elusive. In this study, we investigated Cdc42 activation (Cdc42-GTP), dynamics and role during mouse oocyte meiotic divisions. We show that Cdc42-GTP accumulates in restricted cortical regions overlying meiotic chromosomes or chromatids, in a Ran-GTP-dependent manner. This polarized activation of Cdc42 is required for the recruitment of N-WASP and the formation of F-actin-rich protrusions during polar body formation. Cdc42 inhibition in MII oocytes resulted in the release of N-WASP into the cytosol, a loss of the polarized F-actin cap, and a failure to protrude the second polar body. Cdc42 inhibition also resulted in central spindle defects in activated MII oocytes. In contrast, emission of the first polar body during oocyte maturation could occur in the absence of a functional Cdc42/N-WASP pathway. Therefore, Cdc42 is a new protagonist in chromatin-induced cortical polarization in mammalian oocytes, with an essential role in meiosis II completion, through the recruitment and activation of N-WASP, downstream of the chromatin-centered Ran-GTP gradient.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.